Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1992 Jun;99(2):681-5. doi: 10.1104/pp.99.2.681.
Photooxidative destruction of chloroplasts by exposure of norflurazon-treated cucumber (Cucumis sativus L.) seedlings to white light leads to reduced levels of the nuclear-encoded, peroxisomal enzyme hydroxypyruvate reductase. The partial reduction in hydroxypyruvate reductase activity under photooxidative conditions is accompanied by reductions in levels of hydroxypyruvate reductase protein and transcript. The low level of hydroxypyruvate reductase gene expression in the dark is not affected by norflurazon, and nonphotooxidizing far-red light is able to induce significant increases in hydroxypyruvate reductase expression even in the presence of norflurazon. We conclude that intact plastids are required for maximal expression of hydroxypyruvate reductase in the light and that the plastids affect hydroxypyruvate reductase gene expression at a pretranslational level.
光氧化破坏经 norflurazon 处理的黄瓜（Cucumis sativus L.）幼苗中的叶绿体，导致核编码的过氧化物酶体酶羟丙酮酸还原酶水平降低。在光氧化条件下，羟丙酮酸还原酶活性的部分降低伴随着羟丙酮酸还原酶蛋白和转录本水平的降低。黑暗中羟丙酮酸还原酶基因表达水平较低不受 norflurazon 影响，非光氧化远红光甚至在 norflurazon 存在的情况下也能诱导羟丙酮酸还原酶表达的显著增加。我们得出结论，完整的质体是在光照下羟丙酮酸还原酶最大表达所必需的，并且质体在翻译前水平上影响羟丙酮酸还原酶基因表达。
