[高效液相色谱法测定远志的指纹图谱]
[Fingerprint of Polygala tenuifolia by high performance liquid chromatography].
作者信息
Jiang Yong, Zhang Na, Cui Zhen, Tu Peng-fei
机构信息
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
出版信息
Yao Xue Xue Bao. 2006 Feb;41(2):179-83.
AIM
To study and establish the fingerprint of Polygala tenuifolia by RP-HPLC.
METHODS
The fingerprint of P. tenuifolia was built by using Kromasil C18 as column and acetonitrile-0.05% phosphoric acid aqueous in gradient as mobile phase. The flow rate was 1.0 mL x min(-1), and the detecting wavelength was set at 318 nm. Total 14 batches of P. tenuifolia from different habitats were detected, and different commodities and preparative methods were compared.
RESULTS
Samples from different habitats were of high similarities, and the qualities of "yuanzhitong" and "yuanzhirou" were better than that of "yuanzhigun", while the fingerprints of P. tenuifolia with different preparative methods have not obvious differences. Moreover, the standard fingerprint of P. tenuifolia was originated from the "Computer Aided Similarity Evaluation" software, and 29 common peaks existed in the fingerprint. Each peak in the fingerprint was separated very well under the above chromatographic condition, with good accuracy, stability and repeatability, satisfied to the requests of fingerprint.
CONCLUSION
The RP-HPLC fingerprint method can be used in the quality control of P. tenuifolia.
目的
采用反相高效液相色谱法（RP-HPLC）研究并建立远志的指纹图谱。
方法
以Kromasil C18为色谱柱，乙腈-0.05%磷酸水溶液为流动相进行梯度洗脱，建立远志指纹图谱。流速为1.0 mL·min⁻¹，检测波长设定为318 nm。对14批来自不同产地的远志进行检测，并比较不同商品规格及炮制方法。
结果
不同产地的样品相似度较高，“远志筒”和“远志肉”的质量优于“远志棍”，不同炮制方法的远志指纹图谱无明显差异。此外，远志标准指纹图谱由“计算机辅助相似度评价”软件生成，指纹图谱中有29个共有峰。在上述色谱条件下，指纹图谱中的各峰分离度良好，具有良好的精密度、稳定性和重复性，符合指纹图谱的要求。
结论
RP-HPLC指纹图谱方法可用于远志的质量控制。
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