[建立用于杠柳皮质量评价的反相高效液相色谱指纹图谱]
[Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae].
作者信息
Li Dan-yi, Wang Ting-ting, Chen Xiao-hui, Bi Kai-shun
机构信息
School of Pharmacy, Shenyang Pharmaceutical University, China.
出版信息
Zhong Yao Cai. 2006 Jul;29(7):715-7.
OBJECTIVE
To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae.
METHODS
HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined.
RESULTS
The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by "Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004".
CONCLUSION
The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently.
目的
建立香加皮的RP-HPLC指纹图谱分析方法以控制其质量。
方法
研究香加皮的HPLC指纹图谱及分析方法。采用Kromasil C18柱（250 mm×4.6 mm，5μm），以乙腈和0.025%磷酸盐溶液的混合液为流动相，梯度洗脱。流速为1.0 ml/min。检测波长为220 nm。测定了15批香加皮。
结果
通过聚类分析将15个样品分为4类，并确定了6个优质产地样品建立共有模式。采用“中药色谱指纹图谱相似度评价系统2004版”对样品质量进行评价。
结论
该方法简便可靠。可方便地用于评价和控制香加皮的质量。
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