Xie J, Xue Q, Wang L, Huang Y, Wang J
Institute of Basic Medical Sciences, CAMS, Beijing.
Chin Med Sci J. 1991 Dec;6(4):217-22.
The present study was designed to investigate the effect of hypoxia on the pulmonary adrenoceptors in rats. The density and distribution of pulmonary adrenoceptors were studied by using radioligand binding techniques and autoradiography. The beta adrenoceptors in lung homogenates were found to decrease by 24 h of hypoxia, and to unexpectedly increase at 1 week of hypoxia. Exposure to hypoxia for 2 and 4 weeks led to another decrease in beta adrenoceptors. In contrast, the alpha 1 adrenoceptors were increased markedly at 24 h and 4 weeks of hypoxia. A somewhat higher density of alpha 1 adrenoceptors was also found at 1 week and 7 weeks of hypoxia. The affinity of beta and alpha 1 adrenoceptors did not change. Using a computer image analysis system, beta adrenoceptors were found to be present in higher density in bronchioles than in pulmonary blood vessels before and during hypoxia, while the reverse was true of alpha 1 adrenoceptors. Both beta and alpha 1 adrenoceptors were found in high densities in the parenchyma.
本研究旨在探讨缺氧对大鼠肺肾上腺素能受体的影响。采用放射性配体结合技术和放射自显影术研究肺肾上腺素能受体的密度和分布。发现缺氧24小时时肺匀浆中的β肾上腺素能受体减少，而在缺氧1周时意外增加。缺氧2周和4周导致β肾上腺素能受体再次减少。相反，缺氧24小时和4周时α1肾上腺素能受体明显增加。在缺氧1周和7周时也发现α1肾上腺素能受体密度略高。β和α1肾上腺素能受体的亲和力没有变化。使用计算机图像分析系统，发现在缺氧前和缺氧期间，细支气管中β肾上腺素能受体的密度高于肺血管，而α1肾上腺素能受体则相反。在实质组织中发现β和α1肾上腺素能受体的密度都很高。
