Yi J D, Tian Y, Lu B Z
Institute of Basic Medical sciences of Beijing.
Zhonghua Jie He He Hu Xi Za Zhi. 1993 Feb;16(1):15-7, 61.
With the radioligand binding assay, the maximal binding capacity (Bmax) and affinity (Kd) of alpha, beta-adrenoceptors(alpha AR,beta AR) in the lung membrane and glucocorticoid receptor (GCR) in the lung cytoplasma of rats with experimental respiratory distress syndrome (RDS) induced by oleic acid have been measured. The results demonstrated that the content of alpha AR in rat lungs increased continuously during the experiment, the Bmax at 1st, 4th and 6th hour after oleic acid injection were 139 +/- 40, 127 +/- 12, 116 +/- 25 fmol/mg protein, significantly higher than normal value (83 +/- 7, n = 8-10, P < 0.01). Meanwhile, the content of beta AR and GCR decreased continuously, the Bmax at the same time were 364 +/- 18, 307 +/- 55, 240 +/- 66 and 146 +/- 28, 153 +/- 37, 150 +/- 32 fmol/mg protein respectively, significantly lower than their normal value (490 +/- 61, 227 +/- 14 fmol/mg protein, n = 6-10, P < 0.01). The results indicate that the changes of these receptors may be of significance in the pathogenesis of ARDS.
采用放射性配体结合分析法，测定了油酸诱导的实验性呼吸窘迫综合征（RDS）大鼠肺膜中α、β肾上腺素能受体（αAR、βAR）以及肺细胞质中糖皮质激素受体（GCR）的最大结合容量（Bmax）和亲和力（Kd）。结果显示，实验过程中大鼠肺组织αAR含量持续增加，油酸注射后第1、4、6小时的Bmax分别为139±40、127±12、116±25fmol/mg蛋白，显著高于正常值（83±7，n = 8 - 10，P < 0.01）。同时，βAR和GCR含量持续降低，同一时间的Bmax分别为364±18、307±55、240±66和146±28、153±37、150±32fmol/mg蛋白，显著低于其正常值（490±61、227±14fmol/mg蛋白，n = 6 - 10，P < 0.01）。结果表明，这些受体的变化可能在ARDS发病机制中具有重要意义。
