基于结构和油类型的乳液佐剂功效。

Structure- and oil type-based efficacy of emulsion adjuvants.

作者信息

Jansen Theo, Hofmans Marij P M, Theelen Marc J G, Manders Frans, Schijns Virgil E J C

机构信息

Department of Vaccine Technology & Immunology R&D, The Netherlands.

出版信息

Vaccine. 2006 Jun 29;24(26):5400-5. doi: 10.1016/j.vaccine.2006.03.074. Epub 2006 Apr 17.

DOI:10.1016/j.vaccine.2006.03.074

PMID:16675072

Abstract

Oil-based emulsions are well-known immunopotentiators for inactivated, "killed" vaccines. We addressed the relationship between emulsion structure and levels of in vivo antibody formation to inactivated New Castle Disease virus (NDV) and Infectious Bronchitis virus (IBV) as antigens in 3-week-old chickens. The use of a polymeric emulsifier allowed for direct comparison of three types of emulsions, water-in-oil (W/O), oil-in-water (O/W) and W/O-in-water (W/O/W), while maintaining an identical content of components for each vehicle. They were prepared with either non-metabolizable, mineral oil or metabolizable, Miglyol 840. In addition, we assessed the inherent release capacity of each emulsion variant in vitro. Remarkably, we noted that W/O-type emulsions induced the best immune responses, while they released no antigen during 3 weeks. In general, mineral oil vaccines showed superior efficacy compared to Miglyol 840-based vaccines.

摘要

油基乳剂是灭活“死”疫苗中众所周知的免疫增强剂。我们研究了乳剂结构与3周龄鸡体内针对灭活新城疫病毒（NDV）和传染性支气管炎病毒（IBV）作为抗原的抗体形成水平之间的关系。使用聚合物乳化剂可以直接比较三种类型的乳剂，即油包水（W/O）、水包油（O/W）和水包油包水（W/O/W），同时保持每种载体的成分含量相同。它们用不可代谢的矿物油或可代谢的Miglyol 840制备。此外，我们评估了每种乳剂变体在体外的固有释放能力。值得注意的是，我们发现W/O型乳剂诱导了最佳免疫反应，而它们在3周内没有释放抗原。总体而言，矿物油疫苗比基于Miglyol 840的疫苗显示出更高的效力。