Agola L E, Mburu D N, DeJong R J, Mungai B N, Muluvi G M, Njagi E N M, Loker E S, Mkoji G M
Centre for Biotechnology Research and Development, Kenya Medical Research Institute, P.O. Box 54840, Nairobi 00200, Kenya.
Infect Genet Evol. 2006 Nov;6(6):484-90. doi: 10.1016/j.meegid.2006.03.002. Epub 2006 May 3.
Genetic diversity and population structure of seven populations of Schistosoma mansoni sampled in Kenya were assessed using five microsatellite markers. The mean number of alleles per locus, expected heterozygosity in Hardy-Weinberg equilibrium and pairwise F(ST) values ranged from 5.2 to 10.7, 0.5-0.8 and 3.6-27.3%, respectively. These data reveal that S. mansoni populations in Kenyan have relatively high levels of genetic diversity and is significantly differentiated. Our data combined with information on biogeography support the hypothesis that the strong genetic structure in Kenyan schistosomes is as a result of limited gene flow and large population sizes. Resistance to anthelminthics has not been reported among the Kenyan schistosomes, we hypothesize that this is probably due to the very little gene flow among populations, thereby limiting opportunities for the spread of rare alleles that might confer resistance to the drugs.
利用五个微卫星标记评估了在肯尼亚采集的七个曼氏血吸虫种群的遗传多样性和种群结构。每个位点的平均等位基因数、哈迪-温伯格平衡中的预期杂合度以及成对F(ST)值分别在5.2至10.7、0.5 - 0.8和3.6 - 27.3%之间。这些数据表明，肯尼亚的曼氏血吸虫种群具有相对较高的遗传多样性水平，且存在显著分化。我们的数据与生物地理学信息相结合，支持了这样一种假设，即肯尼亚血吸虫中强大的遗传结构是由于基因流动有限和种群规模较大所致。在肯尼亚血吸虫中尚未有抗蠕虫药耐药性的报道，我们推测这可能是由于种群间基因流动极少，从而限制了可能赋予药物耐药性的稀有等位基因传播的机会。
