手工分枝杆菌生长指示管和Etest与比例法用于结核分枝杆菌药敏试验的比较。
Comparison of the manual Mycobacteria Growth Indicator tube and the Etest with the method of proportion for susceptibility testing of Mycobacterium tuberculosis.
作者信息
Fegou E, Jelastopulu E, Nicolaou S, Sevdali M, Anagnostou S, Kanavaki S, Dimitracopoulos G, Spiliopoulou I
机构信息
Department of Microbiology, University of Patras, School of Medicine, Patras, Greece.
出版信息
Chemotherapy. 2006;52(4):174-7. doi: 10.1159/000093035. Epub 2006 May 2.
BACKGROUND
Clinical microbiology laboratories should provide reliable results on susceptibility testing of Mycobacterium tuberculosis to different agents.
METHODS
The manual Mycobacteria Growth Indicator Tube (MGIT) and Etest were compared to the method of proportion (MOP) for susceptibility testing of 88 clinical isolates of M. tuberculosis against isoniazid (INH), rifampin (RIF), streptomycin (STR) and ethambutol (EMB). Isolates were recovered from different patients and were identified at species level by PCR and hybridization.
RESULTS
Resistance to INH was detected in 20.5, 29.5 and 12.5% of the isolates, followed by STR resistance (19.3, 26.1 and 1.1%), RIF (9.1, 4.5 and 5.7%) and EMB (2.3, 11.4 and 2.3%) by the MOP, MGIT and Etest, respectively. Sensitivity of the manual MGIT ranged from 37.5% for RIF resistance to 100% for EMB, while Etest sensitivity ranged from 5.9% for STR to 62.5% for RIF.
CONCLUSIONS
MOP remains the method of choice, with the manual MGIT showing superior sensitivity at detecting resistance to INH, STR and EMB compared to the Etest.
背景
临床微生物实验室应提供结核分枝杆菌对不同药物敏感性检测的可靠结果。
方法
将手工分枝杆菌生长指示管（MGIT）和Etest与比例法（MOP）进行比较，以检测88株临床分离的结核分枝杆菌对异烟肼（INH）、利福平（RIF）、链霉素（STR）和乙胺丁醇（EMB）的敏感性。分离株来自不同患者，并通过PCR和杂交在种水平上进行鉴定。
结果
通过MOP、MGIT和Etest检测，分别有20.5%、29.5%和12.5%的分离株对INH耐药，其次是对STR耐药（19.3%、26.1%和1.1%）、对RIF耐药（9.1%、4.5%和5.7%）以及对EMB耐药（2.3%、11.4%和2.3%）。手工MGIT的敏感性范围为对RIF耐药为37.5%至对EMB为100%，而Etest的敏感性范围为对STR为5.9%至对RIF为62.5%。
结论
MOP仍然是首选方法，与Etest相比，手工MGIT在检测对INH、STR和EMB的耐药性方面显示出更高的敏感性。
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