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[深圳手足口病患者中检测出的肠道病毒71型基因分型分析]

[Genotype analysis of enterovirus type 71 detected from patients with hand-foot-mouth disease in Shenzhen].

作者信息

He Ya-qing, Yang Hong, Li Lin-lin, Tan Jie, Zhou Li, Mao Li-sha, Yang Fan, Liu Jian-jun, Lv Xing

机构信息

Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2008 Aug;29(8):790-3.

PMID:19103116

Abstract

OBJECTIVE

To carry out genotype analysis of enterovirus type 71, detected from hand-foot-mouth disease patients in Shenzhen in 2004.

METHODS

All samples were tested by reverse transcription-polymerase chain reaction (RT-PCR) using EV71 specific primer. The VP1 and VP4 of EV71 were cloned and sequenced. A phylogenetic tree was constructed by comparing the sequences with genogroups A, B and C genotypes using TreeView and PHYLIP software (3.6b).

RESULTS

The VP1 nucleotide sequence of 4 strains isolated from Shenzhen shared 87.8% - 92.0% identity with genogroup C, while the homogeneity of the VP4 nucleotide sequence was between 85.9% - 87.4%. The homogeneity of the VP1 nucleotide sequence with genotypes A and B was between 81.9% - 84.2% and was 80.6% - 85.0% with VP4. Among the 4 strains, the homogeneity of the VP1 nucleotide sequence was between 94.1% - 99.8% and was 100% with VP4 which formed a small group and could denominate EV71 genetype C4.

CONCLUSION

Similar results were obtained from phylogenetic analysis of EV71 based on VP1 and VP4 nucleotide sequence. The four EV71 strains causing epidemic in Shenzhen could be named as C4 subgroups.

摘要

目的

对2004年深圳市手足口病患者中分离出的肠道病毒71型进行基因分型分析。

方法

所有样本采用肠道病毒71型特异性引物，通过逆转录-聚合酶链反应（RT-PCR）进行检测。对肠道病毒71型的VP1和VP4进行克隆及测序。使用TreeView和PHYLIP软件（3.6b版），通过将序列与A、B、C基因群组的基因型进行比较构建系统发育树。

结果

从深圳分离出的4株病毒的VP1核苷酸序列与C基因群组的同源性为87.8% - 92.0%，而VP4核苷酸序列的同源性为85.9% - 87.4%。VP1核苷酸序列与A、B基因型的同源性为81.9% - 84.2%，与VP4的同源性为80.6% - 85.0%。在这4株病毒中，VP1核苷酸序列的同源性为94.1% - 99.8%，与VP4的同源性为100%，形成一个小群组，可命名为肠道病毒71型C4基因型。