Zhang Shao-li, Xu Bian-li, Guo Wan-shen, Chen Li, Wei Hai-yan, DU Yan-hua, Li Xing-le
College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Sep;30(9):938-41.
To sequence the whole-genome of enterovirus 71 (EV71) srtain isolated from patient with hand, foot and mouth in Henan province in 2008.
Eight overlapping clones covering the whole viral genome were obtained by RT-PCR and the sequences were determined by Sanger dideoxg-mediated chain termination method.
Data it showed that the full length of enterovirus 71 (EV71) HENAN08 genome (not including Poly A tail) is 7405 bp. No deletion or insertion was detected in the coding region. There were several deletions and insertions in 5'UTR and 3'UTR regions. In P1 region, HENAN08 strain shared high homology with AnhuiFY08 strain, Zhejiang08 strain and SHZH strains (SHZH98, SHZH03) but low homology with Cox. A16. In P2 and P3 regions, HENAN08 strain shared higher nucleotide homology with Cox. A16 (81.7% and 83.7%) than that with BrCr and TW2086 strains. The phylogenetic analysis based on P1 region demonstrates that HENAN08 strain had the nearest genetic relationship with AnhuiFY and Zhejiang strains (isolated in 2008).
The HENAN08 strain might belong to the same genogroup with AnhuiFY08 and Zhejiang08 strains as C4 gene subtypes.
对2008年从河南省手足口病患者中分离出的肠道病毒71型（EV71）毒株进行全基因组测序。
通过逆转录聚合酶链反应（RT-PCR）获得覆盖整个病毒基因组的8个重叠克隆，并采用桑格双脱氧介导的链终止法测定序列。
数据显示肠道病毒71型（EV71）HENAN08基因组全长（不包括聚腺苷酸尾）为7405 bp。编码区未检测到缺失或插入。5'非翻译区（UTR）和3'UTR区域存在多处缺失和插入。在P1区域，HENAN08毒株与安徽FY08毒株、浙江08毒株和SHZH毒株（SHZH98、SHZH03）具有高度同源性，但与柯萨奇病毒A16同源性较低。在P2和P3区域，HENAN08毒株与柯萨奇病毒A16的核苷酸同源性（分别为81.7%和83.7%）高于与BrCr和TW2086毒株的同源性。基于P1区域的系统发育分析表明，HENAN08毒株与安徽FY和浙江毒株（2008年分离）的遗传关系最近。
HENAN08毒株可能与安徽FY08和浙江08毒株属于同一基因群，为C4基因亚型。
