Kanazashi M, Gomi K, Nagano T, Tanabe T, Arai T, Fukae M
Department of Periodontics and Endodontics, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
J Periodontal Res. 2006 Jun;41(3):193-9. doi: 10.1111/j.1600-0765.2005.00859.x.
Commercially available enamel proteins, such as Emdogain, are clinically used for periodontal regeneration. However, the real mechanisms behind the bioactivities of enamel proteins is still unclear, as enamel proteins have multicomponents. The purpose of this in vivo study was to identify the cementum regeneration-promoting factor in enamel proteins that is clinically used for periodontal regeneration to induce cementum-promotive and osteopromotive activities.
Cementum regeneration, which is an important part of periodontal regeneration, was examined in experimental cavities prepared on a buccal dehiscence model of dogs. The purification of enamel protein with cementum regeneration activity was carried out by gel filtration and ion exchange chromatographies of newly formed secretory enamel.
Cementum regeneration activity was found in the aggregate comprising 13-17-kDa sheath proteins along with a small amount of amelogenins, found in the newly formed secretory enamel. In these proteins, cementum regeneration activity was detected upon application of the 17-kDa sheath protein, but not by other lower molecular-weight sheath proteins and amelogenins. However, the purified 17-kDa sheath protein induced cementum regeneration activity only in a small area, although the regenerated cementum was thick. The activity of the 17-kDa sheath protein was believed not to have been a result of contamination by growth factors such as transforming growth factor-beta1 (TGF-beta1) found in the enamel protein, as the application of TGF-beta1 induced weak cementum regeneration activity.
It is concluded that the 17-kDa sheath protein itself exhibits cementum regeneration activity, although other factors may be needed to demonstrate its full ability.
市售的釉质蛋白，如Emdogain，临床上用于牙周组织再生。然而，由于釉质蛋白具有多种成分，其生物活性背后的真正机制仍不清楚。本体内研究的目的是确定临床上用于牙周组织再生的釉质蛋白中促进牙骨质再生的因子，以诱导牙骨质促进和骨促进活性。
在犬颊侧骨缺损模型制备的实验腔中检测牙周组织再生的重要组成部分——牙骨质再生情况。通过对新形成的分泌性釉质进行凝胶过滤和离子交换色谱法，对具有牙骨质再生活性的釉质蛋白进行纯化。
在新形成的分泌性釉质中发现，由13 - 17 kDa的鞘蛋白与少量釉原蛋白组成的聚集体具有牙骨质再生活性。在这些蛋白质中，应用17 kDa的鞘蛋白时检测到牙骨质再生活性，而其他低分子量鞘蛋白和釉原蛋白则未检测到。然而，纯化的17 kDa鞘蛋白仅在小面积诱导牙骨质再生活性，尽管再生的牙骨质较厚。17 kDa鞘蛋白的活性被认为不是由釉质蛋白中发现的生长因子如转化生长因子-β1（TGF-β1）污染所致，因为应用TGF-β1诱导的牙骨质再生活性较弱。
得出结论，17 kDa鞘蛋白本身具有牙骨质再生活性，尽管可能需要其他因素来充分发挥其能力。
