Bone Research Unit, Medical Research Council, University of Witwatersrand, Johannesburg, South Africa.
J Periodontal Res. 2012 Jun;47(3):336-44. doi: 10.1111/j.1600-0765.2011.01438.x. Epub 2011 Dec 6.
Binary applications of recombinant human osteogenic protein-1 (hOP-1) and transforming growth factor-β3 (hTGF-β3) synergize to induce pronounced bone formation. To induce periodontal tissue regeneration, binary applications of hOP-1 and hTGF-β(3) were implanted in Class II furcation defects of the Chacma baboon, Papio ursinus.
Defects were created bilaterally in the furcation of the first and second mandibular molars of three adult baboons. Single applications of 25 μg hOP-1 and 75 μg hTGF-β(3) in Matrigel(®) matrix were compared with 20:1 binary applications, i.e. 25 μg hOP-1 and 1.25 μg hTGF-β(3). Morcellated fragments of autogenous rectus abdominis striated muscle were added to binary applications. Sixty days after implantation, the animals were killed and the operated tissues harvested en bloc. Undecalcified sections were studied by light microscopy, and regenerated tissue was assessed by measuring volume and height of newly formed alveolar bone and cementum.
The hOP-1 and hTGF-β(3) induced periodontal tissue regeneration and cementogenesis. Qualitative morphological analysis of binary applications showed clear evidence for considerable periodontal tissue regeneration. Quantitatively, the differences in the histomorphometric values did not reach statistical significance for the group size chosen for this primate study. The addition of morcellated muscle fragments did not enhance tissue regeneration. Binary applications showed rapid expansion of the newly formed bone against the root surfaces following fibrovascular tissue induction in the centre of the treated defects.
Binary applications of hOP-1 and hTGF-β(3) in Matrigel(®) matrix in Class II furcation defects of P. ursinus induced substantial periodontal tissue regeneration, which was tempered, however, by the anatomy of the furcation defect model, which does not allow for the rapid growth and expansion of the synergistic induction of bone formation, particularly when additionally treated with responding myoblastic stem cells.
重组人骨形成蛋白-1（hOP-1）和转化生长因子-β3（hTGF-β3）的二元应用协同诱导明显的骨形成。为了诱导牙周组织再生，将 hOP-1 和 hTGF-β(3) 的二元应用植入 Chacma 狒狒（Papio ursinus）的 II 类分叉缺损中。
在 3 只成年狒狒的第一和第二下颌磨牙的分叉处双侧创建缺损。在 Matrigel（®）基质中比较了 25 μg hOP-1 和 75 μg hTGF-β(3)的单一应用与 20:1 的二元应用，即 25 μg hOP-1 和 1.25 μg hTGF-β(3)。二元应用中添加了切碎的自体腹直肌横纹肌。植入 60 天后，处死动物并整块采集手术组织。对未经脱钙的切片进行光镜研究，并通过测量新形成的牙槽骨和牙骨质的体积和高度来评估再生组织。
hOP-1 和 hTGF-β(3)诱导了牙周组织再生和牙骨质形成。二元应用的定性形态学分析清楚地表明了相当多的牙周组织再生的证据。定量分析，在所选择的用于该灵长类动物研究的组大小下，组织再生的组织形态计量值差异没有达到统计学意义。添加切碎的肌肉片段并没有增强组织再生。二元应用在中心治疗缺陷的纤维血管组织诱导后，迅速使新形成的骨扩展到根表面。
在 P. ursinus 的 II 类分叉缺损中，在 Matrigel（®）基质中 hOP-1 和 hTGF-β(3)的二元应用诱导了大量的牙周组织再生，但由于分叉缺损模型的解剖结构，这种再生受到限制，该模型不允许协同诱导的骨形成快速生长和扩展，尤其是当用反应性成肌细胞进一步治疗时。
