急性和慢性输血后非甲非乙型肝炎中针对丙型肝炎病毒抗原的IgM抗体反应。

IgM-antibody response to hepatitis C virus antigens in acute and chronic post-transfusion non-A, non-B hepatitis.

作者信息

Chau K H, Dawson G J, Mushahwar I K, Gutierrez R A, Johnson R G, Lesniewski R R, Mattsson L, Weiland O

机构信息

Experimental Biology Research, Abbott Laboratories, North Chicago, IL.

出版信息

J Virol Methods. 1991 Dec;35(3):343-52. doi: 10.1016/0166-0934(91)90075-b.

DOI:10.1016/0166-0934(91)90075-b

PMID:1667792

Abstract

A specific IgM solid-phase enzyme-linked immunoassay for the diagnosis of a recent infection by hepatitis C virus (HCV) was developed. The assay utilizes a structural antigen encoded by sequences at the 5' end of HCV (core region) and non-structural (NS) antigens encoded by the NS-3 (33c) and NS-4 (c100-3) regions of the HCV genome. Serial serum samples from several clinically diagnosed post-transfusion non-A, non-B hepatitis patients were analyzed for anti-HCV IgM. This antibody was frequently but transiently detected. Anti-HCV core IgM was more frequently detected than anti-c100-3 or anti-33c IgM. In individuals who resolved their HCV infection or progressed to chronicity, anti-HCV IgM was produced transiently at or near the onset of clinically diagnosed acute hepatitis.

摘要

开发了一种用于诊断近期丙型肝炎病毒（HCV）感染的特异性IgM固相酶联免疫测定法。该测定法利用HCV 5'端序列（核心区域）编码的结构抗原以及HCV基因组NS-3（33c）和NS-4（c100-3）区域编码的非结构（NS）抗原。对来自几名临床诊断为输血后非甲非乙型肝炎患者的系列血清样本进行了抗HCV IgM分析。该抗体经常但短暂地被检测到。抗HCV核心IgM比抗c100-3或抗33c IgM更频繁地被检测到。在HCV感染已消除或进展为慢性的个体中，抗HCV IgM在临床诊断为急性肝炎发作时或接近发作时短暂产生。