Kim Heechul, Ahn Meejung, Lee Jeeyoung, Moon Changjong, Matsumoto Yoh, Koh Chang Sung, Shin Taekyun
Department of Veterinary Medicine, Cheju National University, Jeju 690-756, South Korea.
Neurosci Lett. 2006 Jul 10;402(1-2):76-80. doi: 10.1016/j.neulet.2006.04.008. Epub 2006 May 5.
The expression of phospho-specific caveolin-1 (p-caveolin-1) was analyzed in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis (EAE). Western blot analysis showed that p-caveolin-1 was constitutively expressed in normal spinal cords and that it significantly increased in the spinal cord with EAE at both early and peak stages of EAE (P<0.05), and decreased slightly at the recovery stage of EAE. Immunohistochemistry showed that p-caveolin-1 was constitutively expressed in few vascular endothelial cells and glial cells in the spinal cords of normal rats. In EAE lesions, p-caveolin-1 was intensely immunostained in inflammatory T cells and macrophages. Therefore, we postulate that phosphorylation of caveolin-1 occurred in the inflammatory cells of EAE lesions, and that caveolin-associated cell activation is mainly associated with inflammatory cells that appear during early and peak stages of EAE.
在患有实验性自身免疫性脑脊髓炎（EAE）的Lewis大鼠脊髓中分析了磷酸化特异性小窝蛋白-1（p-小窝蛋白-1）的表达。蛋白质免疫印迹分析表明，p-小窝蛋白-1在正常脊髓中组成性表达，并且在EAE的早期和高峰期，其在脊髓中的表达显著增加（P<0.05），而在EAE的恢复阶段略有下降。免疫组织化学显示，p-小窝蛋白-1在正常大鼠脊髓中的少数血管内皮细胞和神经胶质细胞中组成性表达。在EAE病变中，p-小窝蛋白-1在炎性T细胞和巨噬细胞中呈强免疫染色。因此，我们推测小窝蛋白-1的磷酸化发生在EAE病变的炎性细胞中，并且小窝蛋白相关的细胞活化主要与EAE早期和高峰期出现的炎性细胞有关。
