Kim Heechul, Moon Changjong, Kim Jeongtae, Ahn Meejung, Hyun Jin Won, Park Jae Woo, Kim Sung Ho, Kim Seungjoon, Shin Taekyun
Department of Veterinary Medicine, College of Applied Life Sciences, Cheju National University, Jeju 690-756, Korea.
J Vet Sci. 2007 Dec;8(4):323-7. doi: 10.4142/jvs.2007.8.4.323.
Phosphorylation of caveolin-1 occurs during cell activation by various stimuli. In this study, the involvement of caveolin-1 in an irradiation injured spinal cord was examined by analyzing the phosphorylation of caveolin-1 in the spinal cord of rats after irradiation with a single dose of 15 Gray from a (60)Co gamma-ray source at 24 h post-irradiation (PI). A Western blot analysis showed that the phosphorylated form of caveolin-1 (p-caveolin-1) was expressed constitutively in the normal spinal cords and was significantly higher in the spinal cord of irradiated rats at 24 h PI. The increased expression of ED1, which is a marker of activated microglia/macrophages, was matched with that of p-caveolin-1. In the irradiated spinal cords, there was a higher level of p-caveolin-1 immunoreactivity in the isolectin B4-positive microglial, ependymal, and vascular endothelial cells, in which p-caveolin-1 was weakly and constitutively expressed in the normal control spinal cords. These results suggest that total body irradiation induces activation of microglial cells in the spinal cord through the phosphorylation of caveolin-1.
在各种刺激导致的细胞激活过程中会发生小窝蛋白-1的磷酸化。在本研究中，通过分析单次接受来自（60）Coγ射线源15格雷剂量照射后24小时（照射后）大鼠脊髓中小窝蛋白-1的磷酸化情况，来检测小窝蛋白-1在辐射损伤脊髓中的作用。蛋白质免疫印迹分析显示，小窝蛋白-1的磷酸化形式（p-小窝蛋白-1）在正常脊髓中组成性表达，且在照射后24小时的照射大鼠脊髓中显著升高。作为活化小胶质细胞/巨噬细胞标志物的ED1表达增加与p-小窝蛋白-1的表达增加相匹配。在照射后的脊髓中，异凝集素B4阳性的小胶质细胞、室管膜细胞和血管内皮细胞中的p-小窝蛋白-1免疫反应性水平较高，而在正常对照脊髓中p-小窝蛋白-1在这些细胞中呈弱组成性表达。这些结果表明，全身照射通过小窝蛋白-1的磷酸化诱导脊髓中小胶质细胞的激活。
