Department of Veterinary Anatomy, College of Veterinary Medicine, Veterinary Medical Research Institute, Jeju National University, 1 Ara-1-Dong, Jeju, Jeju 690-756, South Korea.
Brain Res. 2009 Dec 22;1304:155-63. doi: 10.1016/j.brainres.2009.09.060. Epub 2009 Sep 23.
Following stress or inflammation, the 27-kDa heat shock protein (HSP27) is induced in various cell types, where it promotes cell survival and inhibits inflammatory reactions. We examined the expression of HSP27 and phosphorylated HSP27 (p-HSP27) in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis (EAE). Western blotting analysis revealed low levels of HSP27 and p-HSP27 in the normal spinal cords and significantly higher levels in EAE-affected spinal cords. Immunohistochemistry revealed that HSP27 was expressed constitutively in the neurons and some fibrous astrocytes of the spinal cords of normal rats. However, in EAE-affected spinal cords, HSP27 immunoreactivity was higher and located primarily in the fibrous astrocytes of the white matter, whereas few of the inflammatory cells were immunopositive for HSP27. Immunoreactivity for p-HSP27 was detected predominantly in the fibrous astrocytes of the normal controls and was markedly increased in EAE-affected spinal cords. Therefore, the levels of HSP27 expression and phosphorylation of HSP27 were increased primarily during reactive astrogliosis of spinal white matter affected by EAE. These observations suggest that in rat EAE, the increased expression and elevated activation of HSP27 modulate host cell activity, survival, and inflammation to counter the autoimmune inflammatory injury. Our results also suggest that HSP27 plays a role in spontaneous recovery from EAE-induced paralysis.
在应激或炎症后，27kDa 热休克蛋白（HSP27）在各种细胞类型中被诱导，从而促进细胞存活并抑制炎症反应。我们检测了实验性自身免疫性脑脊髓炎（EAE）大鼠脊髓中 HSP27 和磷酸化 HSP27（p-HSP27）的表达。Western blot 分析显示，正常脊髓中 HSP27 和 p-HSP27 的水平较低，而 EAE 受累脊髓中的水平明显较高。免疫组织化学显示，HSP27 在正常大鼠脊髓的神经元和一些纤维性星形胶质细胞中持续表达。然而，在 EAE 受累的脊髓中，HSP27 免疫反应性更高，主要位于白质的纤维性星形胶质细胞中，而炎症细胞中很少有 HSP27 免疫阳性。p-HSP27 的免疫反应性主要在正常对照的纤维性星形胶质细胞中检测到，在 EAE 受累的脊髓中明显增加。因此，HSP27 的表达水平和 HSP27 的磷酸化在 EAE 影响的脊髓白质反应性星形胶质细胞增生中主要增加。这些观察结果表明，在大鼠 EAE 中，HSP27 的表达增加和激活增加调节宿主细胞的活性、存活和炎症，以对抗自身免疫性炎症损伤。我们的结果还表明，HSP27 在自发缓解 EAE 引起的瘫痪中起作用。
