Voytenko A V, Kanbar T, Alber J, Lämmler C, Weiss R, Prenger-Berninghoff E, Zschöck M, Akineden O, Hassan A A, Dmitrenko O A
Kovalenko Research Institute of Experimental Veterinary Medicine, Kurskaya Str. 4, 308002 Belgorod, Russia.
Vet Microbiol. 2006 Aug 25;116(1-3):211-6. doi: 10.1016/j.vetmic.2006.03.009. Epub 2006 May 5.
A species specific PCR test, based on manganese-dependent superoxide dismutase A encoding gene sodA, was developed for the identification of Staphylococcus hyicus, an important bacterial pathogen in pigs. The designed primers allowed a rapid and reliable identification of phenotypically characterized S. hyicus, isolated in Russia, Germany and Denmark. No cross reactivities could be observed investigating staphylococcal reference strains representing 18 different species and subspecies. The use of the described primers might improve a future diagnosis of this bacterial pathogen.
基于依赖锰的超氧化物歧化酶A编码基因sodA，开发了一种用于鉴定猪重要细菌病原体——猪葡萄球菌的种特异性聚合酶链反应（PCR）检测方法。所设计的引物能够快速且可靠地鉴定出在俄罗斯、德国和丹麦分离得到的、具有表型特征的猪葡萄球菌。在研究代表18个不同种和亚种的葡萄球菌参考菌株时，未观察到交叉反应。使用所述引物可能会改善对这种细菌病原体的未来诊断。
