Kanbar T, Voytenko A V, Alber J, Lämmler C, Weiss R, Zschöck M, Shilov I A, Dmitrenko O A, Gintsburg A L
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Str. 107, 35392 Giessen, Germany.
J Vet Med B Infect Dis Vet Public Health. 2006 Nov;53(9):429-33. doi: 10.1111/j.1439-0450.2006.00988.x.
In the present study, previously characterized Staphylococcus hyicus isolated in Russia (n=23) and Germany (n=17) were investigated for the prevalence of the exfoliative toxin encoding genes exhA, exhB, exhC and exhD by multiplex PCR resulting in the detection of exhD positive strains among the S. hyicus isolated from pigs with exudative epidermitis in Russia and the detection of exhC and exhD for one and two strains isolated from exudative epidermitis in Germany respectively. The toxin gene negative strains were generally isolated from apparently healthy pigs, from other animals and from specimens where the relation between the isolation of S. hyicus and the clinical symptoms remained unclear. Partial sequencing of the toxin genes of selected exhC and exhD positive strains and comparing the sequencing results with sequences of exhC and exhD reference strains revealed an almost complete identity. The results of the present study were in agreement with the findings of Andresen and Ahrens (J. Appl. Microbiol., 96, 2004, 1265) and Andresen (J. Vet. Rec., 157, 2005, 376) that the presented multiplex PCR could be used to investigate S. hyicus for toxinogenic potential and that there is an association between the presence of toxin genes in S. hyicus strains from exudative epidermitis. However, comparable with the S. hyicus strains isolated in Germany which were investigated previously by Andresen (J. Vet. Rec., 157, 2005, 376), exhD seems to predominate in S. hyicus strains from Russia.
在本研究中，对先前在俄罗斯（n = 23）和德国（n = 17）鉴定的猪葡萄球菌，通过多重PCR检测其剥脱毒素编码基因exhA、exhB、exhC和exhD的流行情况。结果在俄罗斯从患有渗出性皮炎的猪中分离出的猪葡萄球菌中检测到exhD阳性菌株，在德国从渗出性皮炎中分离出的菌株中分别检测到1株exhC阳性和2株exhD阳性。毒素基因阴性菌株通常从表面健康的猪、其他动物以及猪葡萄球菌分离与临床症状之间关系尚不清楚的标本中分离得到。对选定的exhC和exhD阳性菌株的毒素基因进行部分测序，并将测序结果与exhC和exhD参考菌株的序列进行比较，发现几乎完全相同。本研究结果与Andresen和Ahrens（《应用微生物学杂志》，96，2004，1265）以及Andresen（《兽医记录》，157，2005，376）的研究结果一致，即所提出的多重PCR可用于研究猪葡萄球菌的产毒潜力，并且在患有渗出性皮炎的猪葡萄球菌菌株中，毒素基因的存在之间存在关联。然而，与Andresen（《兽医记录》，157，2005，376）之前研究的德国分离的猪葡萄球菌菌株相比，exhD似乎在俄罗斯的猪葡萄球菌菌株中占主导地位。
