Sarr Jean Birame, Pelleau Stéphane, Toly Cécile, Guitard Juliette, Konaté Lassana, Deloron Philippe, Garcia André, Migot-Nabias Florence
Institut de Recherche pour le Développement (IRD), Unité de Recherche 010: Santé de la mère et de l'enfant en milieu tropical, BP 1386, Dakar, Senegal.
Microbes Infect. 2006 Apr;8(5):1260-8. doi: 10.1016/j.micinf.2005.12.005. Epub 2006 Feb 3.
The evidence of protection afforded by red blood cell polymorphisms against either clinical malaria or Plasmodium falciparum blood levels varies with the study site and the type of malaria transmission. Nevertheless, no clear implication of an antibody-related effect has yet been established in the protection related to red blood cell polymorphisms. We performed a prospective study, where plasma IgG and IgG subclasses directed to recombinant proteins from the merozoite surface protein 2 (MSP2/3D7 and MSP2/FC27) and the ring-infected erythrocyte surface antigen (RESA) were determined in a cohort of 413 Senegalese children before the annual malaria transmission season. The antibody response was dependent on age, and to a lesser extent, on the village of residence. IgG3 responders to all proteins, IgG responders to RESA and MSP2/3D7, as well as IgG2 to RESA and IgG1 responders to MSP2/3D7, presented enhanced mean values of parasite density, as evaluated during an 18-month follow-up. The levels of IgG and IgG3 to MSP2/3D7 were negatively associated with the risk of occurrence of a malaria attack during the following transmission season. Compared to normal children, sickle cell trait carriers presented lower levels of IgG to MSP2/3D7. Similarly, G6PD A- girls had lower levels of IgG and IgG3 to MSP2/FC27 than did G6PD normal girls. The impact of these particular genetic polymorphisms on the modulation of the antibody response is discussed.
红细胞多态性对临床疟疾或恶性疟原虫血中水平所提供的保护证据，因研究地点和疟疾传播类型而异。然而，在与红细胞多态性相关的保护作用中，尚未明确证实存在抗体相关效应。我们进行了一项前瞻性研究，在413名塞内加尔儿童的队列中，于年度疟疾传播季节之前测定了针对裂殖子表面蛋白2（MSP2/3D7和MSP2/FC27）以及环状感染红细胞表面抗原（RESA）重组蛋白的血浆IgG和IgG亚类。抗体反应取决于年龄，在较小程度上还取决于居住村庄。在18个月的随访期间评估发现，对所有蛋白均产生IgG3反应者、对RESA和MSP2/3D7产生IgG反应者，以及对RESA产生IgG2反应和对MSP2/3D7产生IgG1反应者，其寄生虫密度平均值有所升高。针对MSP2/3D7的IgG和IgG3水平与随后传播季节发生疟疾发作的风险呈负相关。与正常儿童相比，镰状细胞性状携带者针对MSP2/3D7的IgG水平较低。同样，G6PD A-女孩针对MSP2/FC27的IgG和IgG3水平低于G6PD正常女孩。本文讨论了这些特定基因多态性对抗体反应调节的影响。
