Sakallioglu A Ebru, Basaran Ozgur, Karakayali Hamdi, Ozdemir B Handan, Yucel Muammer, Arat Zubeyde, Haberal Mehmet
Department of General Surgery, Başkent University, Faculty of Medicine, Ankara, Turkey.
J Burn Care Res. 2006 May-Jun;27(3):357-66. doi: 10.1097/01.BCR.0000216330.93056.06.
This study aimed to clarify the local and systemic immune responses at different burn depths. Thirty female Sprague-Dawley rats were divided into three groups: full-thickness (F), partial-thickness (P), and Sham (S). Burns were induced on three separate areas on the dorsums of rats. Serum levels of interferon (IFN)-gamma; tumor necrosis factor-alpha; interleukin (IL)-1, IL-6, and IL-10 were measured once in controls and 1 hour after burn, 48 hours after burn, and 7 days after burn in F and P groups. Neutrophils, CD68-positive macrophages, HLA-DR-positive cells, and CD3-positive lymphocytes were graded semiquantitatively, and the wounds were examined once in shams and at 1 hour after burn, 48 hours after burn, and 7 days after burn in F and P groups. IL-6 levels were highest in F group, followed by P group 1 hour after burn. IFN-gamma levels were higher in the F group; IL-1 levels were higher in F and P groups at 1 hour after burn. Local accumulation of macrophages was similar in F and P groups. Lymphocytes were denser in P group at 1 hour after burn, and neutrophils were denser in F group at 7 days after burn. We suggest that early elevations of IL-6 and IFN-gamma prolong inflammation in full-thickness burns. Modulation of proinflammatory cytokines may improve burn wound treatment.
本研究旨在阐明不同烧伤深度时的局部和全身免疫反应。将30只雌性Sprague-Dawley大鼠分为三组：全层烧伤组（F）、浅度烧伤组（P）和假手术组（S）。在大鼠背部的三个不同区域造成烧伤。对照组仅检测一次血清干扰素（IFN）-γ、肿瘤坏死因子-α、白细胞介素（IL）-1、IL-6和IL-10水平；F组和P组在烧伤后1小时、48小时和7天各检测一次。对中性粒细胞、CD68阳性巨噬细胞、HLA-DR阳性细胞和CD3阳性淋巴细胞进行半定量分级，假手术组仅检查一次伤口，F组和P组在烧伤后1小时、48小时和7天各检查一次伤口。F组IL-6水平最高，其次是烧伤后1小时的P组。F组IFN-γ水平较高；F组和P组在烧伤后1小时IL-1水平较高。F组和P组巨噬细胞的局部聚集情况相似。烧伤后1小时P组淋巴细胞更密集，烧伤后7天F组中性粒细胞更密集。我们认为，IL-6和IFN-γ的早期升高会延长全层烧伤的炎症反应。调节促炎细胞因子可能会改善烧伤创面的治疗效果。
