Nakamura Satoshi, Hamagami Jun-ichi, Yamashita Kimihiro
Department of Bioceramics, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan.
J Biomed Mater Res B Appl Biomater. 2007 Jan;80(1):102-6. doi: 10.1002/jbm.b.30574.
Carbonate-containing hydroxyapatite (HA) films were prepared by low-temperature hydrothermal annealing from carbonate-containing calcium phosphate amorphous coatings on titanium substrates. The biocompatibility of the carbonate-containing HA layers was estimated by in vitro tests using simulated body fluid (SBF). Precursory amorphous coatings were deposited with rf-magnetron sputtering apparatus, using calcium phosphate glass target in Ar/CO2 atmosphere. The carbonate-containing HA coatings were successfully formed by the annealing at above 130 degrees C for 20 h. On the basis of SEM observation, about 2-microm thickness films coated rigidly were durable enough for the hydrothermal treatment. The coating layer was revealed to consist of single phase of PO4(-) and OH- partially carbonated HA by XRD and IR analyses. Overgrowing of bone-like apatite layers on the carbonate-containing HA surfaces in the SBF implied that the obtained films acquired a sufficient osteoconductivity, while it was still unclear that activity was enhanced, compared to pure HA coatings. The low-temperature hydrothermal annealing method was effective for preparation of rigid HA coatings on titanium as well as modification of their chemical compositions.
通过低温水热退火法，在钛基底上由含碳酸盐的磷酸钙非晶涂层制备了含碳酸盐的羟基磷灰石（HA）薄膜。使用模拟体液（SBF）通过体外试验评估了含碳酸盐的HA层的生物相容性。前驱非晶涂层是在Ar/CO₂气氛中使用磷酸钙玻璃靶，通过射频磁控溅射仪沉积的。通过在130℃以上退火20小时成功形成了含碳酸盐的HA涂层。基于扫描电子显微镜（SEM）观察，刚性涂覆的约2微米厚的薄膜对于水热处理具有足够的耐久性。通过X射线衍射（XRD）和红外光谱（IR）分析表明，涂层由PO₄⁻和OH⁻的单相组成，HA部分碳酸化。在SBF中，含碳酸盐的HA表面上类骨磷灰石层的过度生长意味着所获得的薄膜具有足够的骨传导性，然而，与纯HA涂层相比，其活性是否增强仍不清楚。低温水热退火法对于在钛上制备刚性HA涂层及其化学成分的改性是有效的。
