Poli Annarita, Esposito Enrico, Lama Licia, Orlando Pierangelo, Nicolaus Giancarlo, de Appolonia Francesca, Gambacorta Agata, Nicolaus Barbara
Istituto di Chimica Biomolecolare (ICB), CNR, Via Campi Flegrei 34, 80078 Pozzuoli (Napoli), Italy.
Syst Appl Microbiol. 2006 Jun;29(4):300-7. doi: 10.1016/j.syapm.2005.10.003. Epub 2005 Nov 3.
A new thermophilic spore-forming strain MR3CT was isolated from geothermal soil located on Mount Rittmann in Antarctica. Strain MR3CT was Gram-positive, rod-shaped, occurring in pairs or filamentous. Growth was observed between 45 and 65 degrees C (optimum 61 degrees C) and at pH 5.0-6.5 (optimum pH 5.6). It was capable of utilizing galactose, trehalose, maltose and sucrose. The microorganism produced an exopolysaccharide and synthesized an extracellular constitutive amylolytic activity. The G + C content of DNA was 43.5 mol%. On the basis of 16S rRNA gene sequence similarity, strain MR3CT was shown to be related most closely to Anoxybacillus species. Chemotaxonomic data (major isoprenoid quinone-menaquinone-7; major fatty acid-iso-C15:0 and iso-C17:0) supported the affiliation of strain MR3C1T to the genus Anoxybacillus. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain MR3CT from the validly published Anoxybacillus species. MR3CT therefore represents a new species, for which the name Anoxybacillus amylolyticus sp. nov., is proposed, with the type strain MR3CT (= ATCC BAA-872T = DSM 15939T = CIP 108338T).
从南极洲里特曼山的地热土壤中分离出一株新的嗜热芽孢杆菌菌株MR3CT。菌株MR3CT为革兰氏阳性，杆状，成对或丝状存在。在45至65摄氏度（最适温度61摄氏度）和pH 5.0 - 6.5（最适pH 5.6）条件下可观察到其生长。它能够利用半乳糖、海藻糖、麦芽糖和蔗糖。该微生物产生一种胞外多糖，并合成一种细胞外组成型淀粉分解活性。DNA的G + C含量为43.5 mol%。基于16S rRNA基因序列相似性，菌株MR3CT显示与嗜热栖热放线菌属物种关系最为密切。化学分类学数据（主要类异戊二烯醌 - 甲基萘醌 - 7；主要脂肪酸 - 异C15:0和异C17:0）支持菌株MR3C1T属于嗜热栖热放线菌属。DNA - DNA杂交、生理生化测试结果使得菌株MR3CT与已有效发表的嗜热栖热放线菌属物种在基因型和表型上得以区分。因此，MR3CT代表一个新物种，为此提出名称嗜热栖热淀粉分解放线菌（Anoxybacillus amylolyticus sp. nov.），模式菌株为MR3CT（= ATCC BAA - 872T = DSM 15939T = CIP 108338T）。
