Faculty of Science, Biology Department, Ankara University, Tandogan, Ankara, Turkey.
J Basic Microbiol. 2011 Apr;51(2):136-46. doi: 10.1002/jobm.201000115. Epub 2010 Nov 12.
A novel moderately thermophilic, Gram-positive staining, rod-shaped, spore-forming, motile, facultative anaerobic, and α-glucosidase producing strain A343(T), was isolated from a high temperature well-pipeline sediment sample in Salavatli province of Aydin, Turkey. Growth was observed at 37-69 ºC (optimum 60 °C), at pH 5.5-9.5 (optimum 8.0-9.0) and at salinities from 0 to 4.5% (w/v) (optimum 2%). Strain A343(T) was able to grow on a wide range of carbon sources. Gelatin and starch utilization, amylase, catalase and oxidase activities, reduction of nitrate to nitrite were all positive. The G+C content of the genomic DNA was 45.1 mol%. The major menaquinone was MK-7. The dominant cellular fatty acids were: iso-C15:0, C16:0, and iso-C17:0. The phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain A343(T) belonged to the genus Anoxybacillus. The 16S rRNA gene sequence similarity between strain A343(T) and the type strains of recognized Anoxybacillus species was ranged from 95.8 to 99.4%. DNA-DNA hybridization revealed low homology with its closest relative Anoxybacillus kamchatkensis (49.7%). In addition to the total cell protein profiles, the Rep-PCR and the intergenic 16S-23S rRNA gene fingerprinting profiles differentiated strain A343(T) from all of the reference Anoxybacillus species used. Based upon phenotypic, phylogenetic and chemotaxonomic evidence, strain A343(T) was assigned to a new species within the genus Anoxybacillus, A. salavatliensis sp. nov. (The type strain A343(T) = DSM 22626(T) = NCIMB 14579(T)). The 16S rRNA gene nucleotide sequence of strain A343(T) is available in the GenBank database under the accession number--EU326496. Weinheim).
一株新型中温、革兰氏阳性、杆状、孢子形成、能动、兼性厌氧、产α-葡萄糖苷酶的菌株 A343(T)，是从土耳其艾登省萨拉瓦特利的一口高温油井管道沉积物样品中分离出来的。该菌在 37-69℃（最适 60℃）、pH5.5-9.5（最适 8.0-9.0）和 0-4.5%（w/v）的盐度（最适 2%）下生长。菌株 A343(T) 能够利用广泛的碳源生长。明胶和淀粉利用、淀粉酶、过氧化氢酶和氧化酶活性、硝酸盐还原为亚硝酸盐均为阳性。基因组 DNA 的 G+C 含量为 45.1mol%。主要的menaquinone 是 MK-7。主要的细胞脂肪酸是：iso-C15:0、C16:0 和 iso-C17:0。基于 16S rRNA 基因序列的系统发育分析表明，菌株 A343(T) 属于 Anoxybacillus 属。菌株 A343(T)与已鉴定的 Anoxybacillus 种的模式菌株的 16S rRNA 基因序列相似性在 95.8%至 99.4%之间。DNA-DNA 杂交显示与最接近的亲缘关系 Anoxybacillus kamchatkensis（49.7%）的同源性较低。除了总细胞蛋白图谱外，Rep-PCR 和基因间 16S-23S rRNA 基因指纹图谱也将菌株 A343(T) 与使用的所有参考 Anoxybacillus 种区分开来。基于表型、系统发育和化学分类学证据，菌株 A343(T) 被分配到 Anoxybacillus 属的一个新种，即 Anoxybacillus salavatliensis sp. nov.（模式菌株 A343(T)=DSM 22626(T)=NCIMB 14579(T)）。菌株 A343(T) 的 16S rRNA 基因核苷酸序列已在 GenBank 数据库中注册，登录号为--EU326496。Weinheim）。
