Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan 650091, PR China.
Yunnan Reascend Tobacco Technology (Group) Co., Ltd, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):118-122. doi: 10.1099/ijs.0.020834-0. Epub 2010 Feb 19.
Two novel thermophilic, spore-forming bacterial strains, T-11(T) and E-112(T), were isolated from hot springs in Tengchong and Eryuan counties of Yunnan province in south-west China. The strains were Gram-stain-positive rods, occurring singly or in chains. Growth of strain T-11(T) was observed between 30 and 75 °C (optimum 50 °C) and at pH 7-11 (optimum pH 8.5), while the temperature range for strain E-112(T) was 35-70 °C (optimum 55 °C) and the pH range was 7.0-11.0 (optimum pH 8.0). The DNA G+C contents of strains T-11(T) and E-112(T) were 41.1 and 42.6 mol%, respectively. On the basis of 16S rRNA gene sequence similarity, the two strains were shown to be related most closely to Anoxybacillus species. The chemotaxonomic characteristics [predominant isoprenoid quinone menaquinone 7 (MK-7); major fatty acids iso-C(15 : 0) and iso-C(17 : 0)] also supported the affiliation of strains T-11(T) and E-112(T) to the genus Anoxybacillus. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains T-11(T) and E-112(T) from Anoxybacillus species with validly published names. Strains T-11(T) and E-112(T) therefore represent two novel species, for which the names Anoxybacillus tengchongensis sp. nov. (type strain T-11(T) =CCTCC AB209237(T) =KCTC 13721(T)) and Anoxybacillus eryuanensis sp. nov. (type strain E-112(T) =CCTCC AB209236(T) =KCTC 13720(T)) are proposed.
两株新型嗜热、产芽孢细菌菌株 T-11(T)和 E-112(T)从中国西南部云南省腾冲县和洱源县的温泉中分离得到。这些菌株为革兰氏阳性杆菌，单独或成链存在。菌株 T-11(T)的生长温度范围为 30 至 75°C（最佳温度为 50°C），pH 值范围为 7-11（最佳 pH 值为 8.5），而菌株 E-112(T)的生长温度范围为 35-70°C（最佳温度为 55°C），pH 值范围为 7.0-11.0（最佳 pH 值为 8.0）。菌株 T-11(T)和 E-112(T)的 DNA G+C 含量分别为 41.1 和 42.6mol%。基于 16S rRNA 基因序列相似性，这两株菌与 Anoxybacillus 属的种最为密切相关。化学分类特征[主要异戊烯醌menaquinone 7（MK-7）；主要脂肪酸为 iso-C(15：0)和 iso-C(17：0)]也支持菌株 T-11(T)和 E-112(T)属于 Anoxybacillus 属。DNA-DNA 杂交和生理生化试验的结果表明，菌株 T-11(T)和 E-112(T)在基因型和表型上与已发表有效名称的 Anoxybacillus 种不同。因此，菌株 T-11(T)和 E-112(T)代表两个新种，分别命名为腾冲嗜热杆菌（新种）（模式菌株 T-11(T) =CCTCC AB209237(T) =KCTC 13721(T)）和洱源嗜热杆菌（新种）（模式菌株 E-112(T) =CCTCC AB209236(T) =KCTC 13720(T)）。
