Kondo S, Nishioka K
Department of Dermatology, School of Medicine, Tokyo Medical and Dental University, Japan.
Photodermatol Photoimmunol Photomed. 1991 Oct;8(5):212-7.
The sensitivity of fibroblasts derived from patients with chronic actinic dermatitis (CAD) ultraviolet B (UVB), UVA and superoxide radical was examined. Fibroblasts from the skin of 3 patients with CAD showed abnormal hypersensitivity after exposure to mid-UV (UVB) and near-UV (UVA) radiation in both the dividing and quiescent phases. In their dividing phases, the 3 cell strains (CAD1TO, CAD2TO and CAD3TO) exhibited 78, 55 and 82 J/m2 for the mean lethal dose (Do) values in UVB survival curves. In the quiescent phases their Do values were 262, 226 and 165 J/m2, respectively. Regarding UVA sensitivity, Do value in the dividing phases were 4.9, 3.9 and 3.8 x 10(4) J/m2, and in the quiescent phases, 2.0, 1.9 and 1.7 x 10(5) J/m2. Do values were lower than in the cell strains derived from healthy individuals (Do = 120 +/- 18 and 390 +/- 72 J/m2) for UVB sensitivity in the dividing and quiescent phases and (5.9 +/- 0.6) x 10(4) and (3.2 +/- 1.0) x 10(5) J/m2 for UVA sensitivity in the dividing and quiescent phases. DNA repair synthesis was similar between the cell lines. Furthermore, the 3 CAD cell lines showed a higher sensitivity to superoxide radical generated by hypoxanthine/xanthine oxidase treatment. These results suggest that patients with CAD may suffer from a defect in dealing with damage induced by oxygen radicals.
检测了慢性光化性皮炎（CAD）患者来源的成纤维细胞对紫外线B（UVB）、紫外线A（UVA）和超氧自由基的敏感性。3例CAD患者皮肤来源的成纤维细胞在分裂期和静止期暴露于中波紫外线（UVB）和近紫外线（UVA）辐射后均表现出异常的超敏反应。在分裂期，3个细胞株（CAD1TO、CAD2TO和CAD3TO）在UVB存活曲线中的平均致死剂量（Do）值分别为78、55和82 J/m2。在静止期，它们的Do值分别为262、226和165 J/m2。关于UVA敏感性，分裂期的Do值分别为4.9、3.9和3.8×10(4) J/m2，静止期为2.0、1.9和1.7×10(5) J/m2。对于UVB敏感性，分裂期和静止期的Do值均低于健康个体来源的细胞株（Do = 120±18和390±72 J/m2），对于UVA敏感性，分裂期和静止期分别为(5.9±0.6)×10(4)和(3.2±1.0)×10(5) J/m2。各细胞系之间的DNA修复合成情况相似。此外，3个CAD细胞系对次黄嘌呤/黄嘌呤氧化酶处理产生的超氧自由基表现出更高的敏感性。这些结果表明，CAD患者可能在处理氧自由基诱导的损伤方面存在缺陷。
