Luy H, Frenk E, Applegate L A
Department of Dermatology, University Hospital (CHUV), Lausanne, Switzerland.
Photodermatol Photoimmunol Photomed. 1994 Jun;10(3):126-33.
Abnormal photosensitivity of skin in the photosensitivity dermatitis/actinic reticuloid syndrome (PD/AR) correlating to an abnormal photosensitivity of dermal fibroblasts from these patients has been established previously. Cultured human skin fibroblasts from normal infant and adult donors, foreskin tissue and patients with PD/AR were assayed for oxidative stress induced membrane damage following ultraviolet A (UVA) radiation (340-450 nm) and hydrogen peroxide treatment by measurement of lactate dehydrogenase release. Skin fibroblasts from PD/AR patients were, in general, 2- and 4-fold more sensitive than normal controls regarding membrane-induced damage to UVA radiation and hydrogen peroxide treatment, respectively. Cells from one patient where biopsies were taken during full disease flair and 2 years later, following disease remission by immunosuppressive therapy, showed the same cellular sensitivity. In addition, fibroblasts from foreskin tissue were resistant to UVA radiation as measured by lactate dehydrogenase release, suggesting that this frequently used source of cell culture does not provide the best control group for comparison with photosensitive disease states. All these results suggest that the target for the cellular sensitivity seen in cells from PD/AR patients is the membrane.
光敏性皮炎/光化性类网状细胞增多症（PD/AR）中皮肤的异常光敏感性与这些患者真皮成纤维细胞的异常光敏感性相关，这一点先前已得到证实。对来自正常婴儿和成人供体、包皮组织以及PD/AR患者的培养人皮肤成纤维细胞，通过测量乳酸脱氢酶释放量，检测紫外线A（UVA，340 - 450纳米）辐射和过氧化氢处理后氧化应激诱导的膜损伤。总体而言，PD/AR患者的皮肤成纤维细胞对UVA辐射和过氧化氢处理诱导的膜损伤分别比正常对照敏感2倍和4倍。在疾病完全发作期间进行活检的一名患者，以及2年后在免疫抑制治疗使疾病缓解后进行活检的该患者的细胞，显示出相同的细胞敏感性。此外，通过乳酸脱氢酶释放量测量发现，包皮组织的成纤维细胞对UVA辐射具有抗性，这表明这种常用的细胞培养来源不能为与光敏疾病状态进行比较提供最佳对照组。所有这些结果表明，PD/AR患者细胞中观察到的细胞敏感性靶点是细胞膜。
