Delcomyn Carrie A, Bushway Karen E, Henley Michael V
Applied Research Associates, Inc., Florida, USA.
Environ Sci Technol. 2006 Apr 15;40(8):2759-64. doi: 10.1021/es052146+.
Bleach solutions containing the active ingredient hypochlorite (OCl-) serve as powerful biological disinfectants but are highly caustic and present a significant compatibility issue when applied to contaminated equipment or terrain. A neutral, bicarbonate-buffered aqueous solution of Oxone (2K2HSO5.KHSO4.K2SO4) and sodium chloride that rapidly generates hypochlorite and hypochlorous acid (HOCl) in situ was evaluated as a new alternative to bleach for the inactivation of biological agents. The solution produced a free chlorine (HOCl + OCl-) concentration of 3.3 g/L and achieved > or =5.8-log inactivation of spores of Bacillus atrophaeus, Bacillus thuringiensis, Aspergillus niger, and Escherichia coli vegetative cells in 1 min at 22 degrees C. Seawaterwas an effective substitute for solid sodium chloride and inactivated 5 to 8 logs of each organism in 10 min over temperatures ranging from -5 degrees C to 55 degrees C. Sporicidal effectiveness increased as free chlorine concentrations shifted from OCl- to HOCl. Neutrally buffered Oxone-chloride and Oxone-seawater solutions are mitigation alternatives for biologically contaminated equipment and environments that would otherwise be decontaminated using caustic bleach solutions.
含有活性成分次氯酸盐（OCl-）的漂白剂溶液是强大的生物消毒剂，但腐蚀性很强，应用于受污染的设备或区域时会出现严重的兼容性问题。一种由过一硫酸钾（2K2HSO5.KHSO4.K2SO4）和氯化钠组成的中性、碳酸氢盐缓冲水溶液，能原位快速生成次氯酸盐和次氯酸（HOCl），被评估为一种替代漂白剂用于灭活生物制剂的新选择。该溶液产生的游离氯（HOCl + OCl-）浓度为3.3 g/L，在22℃下1分钟内对萎缩芽孢杆菌、苏云金芽孢杆菌、黑曲霉的孢子以及大肠杆菌营养细胞实现了≥5.8对数级的灭活。海水是固体氯化钠的有效替代品，在-5℃至55℃的温度范围内，10分钟内对每种生物灭活5至8个对数级。随着游离氯浓度从OCl-向HOCl转变，杀孢子效果增强。中性缓冲的过一硫酸钾-氯化物溶液和过一硫酸钾-海水溶液是受生物污染的设备和环境的缓解替代方案，否则这些设备和环境会使用腐蚀性漂白剂溶液进行去污处理。
