Furutani Ayako, Nakayama Takeshi, Ochiai Hirokazu, Kaku Hisatoshi, Kubo Yasuyuki, Tsuge Seiji
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Prefectural University, Kyoto, Japan.
FEMS Microbiol Lett. 2006 Jun;259(1):133-41. doi: 10.1111/j.1574-6968.2006.00265.x.
A regulatory protein HrpXo of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, is known to control the expression of hrp genes that encode components of a type III secretion system and of some effector protein genes. In this study, we screened novel HrpXo regulons from the genome database of X. oryzae pv. oryzae, searching for ORFs preceded by two predicted sequence motifs, a plant-inducible promoter box-like sequence and a -10 box-like sequence. Using a gus reporter system, nine of 15 ORF candidates were expressed HrpXo dependently. We also showed by base-substituted mutagenesis that both motifs are essential for the expression of the genes.
水稻白叶枯病的病原菌——水稻白叶枯病菌中的一种调控蛋白HrpXo，已知可控制编码III型分泌系统组分的hrp基因以及一些效应蛋白基因的表达。在本研究中，我们从水稻白叶枯病菌的基因组数据库中筛选新的HrpXo调控子，寻找前面带有两个预测序列基序（一个植物诱导型启动子盒样序列和一个-10盒样序列）的开放阅读框（ORF）。利用gus报告系统，15个ORF候选序列中有9个的表达依赖于HrpXo。我们还通过碱基取代诱变表明，这两个基序对于这些基因的表达都是必不可少的。
