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稻生黄单胞菌野油菜致病变种 Tal7 转录激活子样效应因子激活水稻基因 Os09g29100 抑制水稻免疫

A Transcription Activator-Like Effector Tal7 of Xanthomonas oryzae pv. oryzicola Activates Rice Gene Os09g29100 to Suppress Rice Immunity.

机构信息

School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban (South) by Ministry of Agriculture, Shanghai, 200240, China.

State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

出版信息

Sci Rep. 2017 Jul 11;7(1):5089. doi: 10.1038/s41598-017-04800-8.

DOI:10.1038/s41598-017-04800-8
PMID:28698641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5505973/
Abstract

Xanthomonas oryzae pv. oryzicola (Xoc) and X. oryzae pv. oryzae (Xoo) cause bacterial leaf streak (BLS) and bacterial leaf blight (BLB) in rice, respectively. Unlike Xoo, endogenous avirulence-resistance (avr-R) gene interactions have not been identified in the Xoc-rice pathosystem; however, both pathogens possess transcription activator-like effectors (TALEs) that are known to modulate R or S genes in rice. The transfer of individual tal genes from Xoc RS105 (hypervirulent) into Xoc YNB0-17 (hypovirulent) led to the identification of tal7, which suppressed avrXa7-Xa7 mediated defense in rice containing an Xa7 R gene. Mobility shift and microscale thermophoresis assays showed that Tal7 bound two EBE sites in the promoters of two rice genes, Os09g29100 and Os12g42970, which encode predicted Cyclin-D4-1 and GATA zinc finger family protein, respectively. Assays using designer TALEs and a TALE-free strain of Xoo revealed that Os09g29100 was the biologically relevant target of Tal7. Tal7 activates the expression of rice gene Os09g29100 that suppresses avrXa7-Xa7 mediated defense in Rice. TALEN editing of the Tal7-binding site in the Os09g29100 gene promoter further enhanced resistance to the pathogen Xoc RS105. The suppression of effector-trigger immunity (ETI) is a phenomenon that may contribute to the scarcity of BLS resistant cultivars.

摘要

黄单胞菌稻生致病变种（Xoc）和稻黄单胞菌稻致病变种（Xoo）分别引起水稻细菌性条斑病（BLS）和细菌性叶斑病（BLB）。与 Xoo 不同，内源性无毒-抗性（avr-R）基因相互作用尚未在 Xoc-水稻病原系统中确定；然而，这两种病原体都拥有转录激活子样效应物（TALEs），已知这些效应物可在水稻中调节 R 或 S 基因。将单个 tal 基因从 Xoc RS105（高毒力）转移到 Xoc YNB0-17（低毒力）中，导致了 tal7 的鉴定，tal7 抑制了含有 Xa7 R 基因的水稻中 avrXa7-Xa7 介导的防御。迁移率变动和微尺度热泳分析表明，Tal7 结合了两个水稻基因 Os09g29100 和 Os12g42970 启动子中的两个 EBE 位点，这两个基因分别编码预测的细胞周期蛋白 D4-1 和 GATA 锌指家族蛋白。使用设计的 TALEs 和一种无 TALE 的 Xoo 菌株进行的试验表明，Os09g29100 是 Tal7 的生物学相关靶标。Tal7 激活了水稻基因 Os09g29100 的表达，该基因抑制了水稻中 avrXa7-Xa7 介导的防御。在 Os09g29100 基因启动子中 Tal7 结合位点的 TALEN 编辑进一步增强了对病原体 Xoc RS105 的抗性。效应物触发免疫（ETI）的抑制是一种可能导致 BLS 抗性品种稀缺的现象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/d6f2af3539c5/41598_2017_4800_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/86f554d2ff44/41598_2017_4800_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/996b5f714cd6/41598_2017_4800_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/e8eb6a7757a2/41598_2017_4800_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/c26d33f6099d/41598_2017_4800_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/e9e95577fb38/41598_2017_4800_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/d6f2af3539c5/41598_2017_4800_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/86f554d2ff44/41598_2017_4800_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/996b5f714cd6/41598_2017_4800_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/e8eb6a7757a2/41598_2017_4800_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/c26d33f6099d/41598_2017_4800_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/e9e95577fb38/41598_2017_4800_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4838/5505973/d6f2af3539c5/41598_2017_4800_Fig6_HTML.jpg

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