Ando Takuya, Ishiguro Hideyuki, Kuwabara Yoshiyuki, Kimura Masahiro, Mitsui Akira, Kurehara Hiroki, Sugito Nobuyoshi, Tomoda Keisuke, Mori Ryota, Takashima Nobuhiro, Ogawa Ryo, Fujii Yoshitaka
Department of Surgery II, Nagoya City University Medical School, 1 Kawasumi, Nagoya 467-8601, Japan.
Oncol Rep. 2006 Jun;15(6):1551-5.
ACP6 (acid phosphatase 6, lysophosphatidic) is a lysophosphatidic acid (LPA)-specific phosphatase that hydrolyzes LPA to monoacylglycerol and is involved in lipid metabolism in the mitochondria. Its role in oncogenesis and cancer progression has not been studied. In this study, we examined the expression of ACP6 mRNA and evaluated its clinical significance in esophageal squamous cell carcinoma (ESCC). Expression of ACP6 mRNA was quantified by real-time reverse transcription polymerase chain reaction using the LightCycler in 70 esophageal ESCC specimens and their paired normal esophageal mucosa. The data were analyzed with reference to clinicopathological factors. ACP6 mRNA expression in esophageal cancer tissue was significantly lower than that in corresponding normal esophageal mucosa (P=0.0301). Among the esophageal cancer tissues, ACP6 mRNA expression significantly correlated with local tumor invasion (T factor, P=0.0461) and lymph node metastasis (P=0.0128). Furthermore, low ACP6 mRNA expression was associated with a significantly shorter survival time compared with high expression (log-rank test, P=0.0358). In multivariate analysis, ACP6 mRNA expression emerged as a significant independent factor (P=0.0148). Impaired ACP6 expression may lead to more aggressive invasion of ESCC, and ACP6 mRNA expression level could be an independent prognostic factor for patients with ESCC.
ACP6（酸性磷酸酶6，溶血磷脂酸）是一种溶血磷脂酸（LPA）特异性磷酸酶，可将LPA水解为单酰甘油，并参与线粒体中的脂质代谢。其在肿瘤发生和癌症进展中的作用尚未得到研究。在本研究中，我们检测了ACP6 mRNA的表达，并评估了其在食管鳞状细胞癌（ESCC）中的临床意义。使用LightCycler通过实时逆转录聚合酶链反应对70例食管ESCC标本及其配对的正常食管黏膜中ACP6 mRNA的表达进行定量。参照临床病理因素对数据进行分析。食管癌组织中ACP6 mRNA的表达明显低于相应的正常食管黏膜（P = 0.0301）。在食管癌组织中，ACP6 mRNA表达与局部肿瘤浸润（T因子，P = 0.0461）和淋巴结转移（P = 0.0128）显著相关。此外，与高表达相比，低ACP6 mRNA表达与显著缩短的生存时间相关（对数秩检验，P = 0.0358）。在多变量分析中，ACP6 mRNA表达是一个显著的独立因素（P = 0.0148）。ACP6表达受损可能导致ESCC更具侵袭性，且ACP6 mRNA表达水平可能是ESCC患者的一个独立预后因素。
