Spm元件的tnpA和tnpD基因产物是烟草转座所必需的。
The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco.
作者信息
Masson P, Strem M, Fedoroff N
机构信息
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.
出版信息
Plant Cell. 1991 Jan;3(1):73-85. doi: 10.1105/tpc.3.1.73.
Abstract
The maize Suppressor-mutator (Spm) element encodes four alternatively spliced transcripts designated tnpA, tnpB, tnpC, and tnpD. tnpA and tnpB are monocistronic, whereas tnpC and tnpD are dicistronic, and the protein-coding sequences of each transcript overlap extensively with those of one or more of the other transcripts. We have analyzed the role of the Spm-encoded gene products in element transposition by using cDNAs with a single open reading frame to (1) complement Spm elements with frameshift mutations and (2) complement each other in a tobacco transposition assay. We report that whereas the tnpA and tnpD gene products are essential for transposition, the tnpB and tnpC gene products are not. We have analyzed the structure of empty donor sites, new insertion sites, and potential transposition intermediates. We discuss the implications of our findings for the mechanism of Spm transposition.
摘要
玉米抑制-突变体（Spm）元件编码四种可变剪接转录本，分别命名为tnpA、tnpB、tnpC和tnpD。tnpA和tnpB是单顺反子，而tnpC和tnpD是双顺反子，并且每个转录本的蛋白质编码序列与一个或多个其他转录本的序列广泛重叠。我们通过使用具有单个开放阅读框的cDNA来分析Spm编码的基因产物在元件转座中的作用：（1）用移码突变体互补Spm元件；（2）在烟草转座试验中相互互补。我们报道，虽然tnpA和tnpD基因产物对转座至关重要，但tnpB和tnpC基因产物并非如此。我们分析了空供体位点、新插入位点和潜在转座中间体的结构。我们讨论了我们的发现对Spm转座机制的意义。
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