Cuypers H, Dash S, Peterson P A, Saedler H, Gierl A
Abteilung Molekulare Pflanzengenetik, Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, FRG.
EMBO J. 1988 Oct;7(10):2953-60. doi: 10.1002/j.1460-2075.1988.tb03157.x.
Genetic and molecular analysis has revealed a specific En-element of deletion derivative (En-I102) which reduces En/Spm-induced mutability. In the presence of En-I102 the excision frequency of both the autonomous En-1 element and the inhibitor element Spm-I5719A is reduced and excision occurs later in development. The 3697 bp long En-I102 element is derived from En-1 by an internal deletion of 4590 bp removing nucleotides 1862-6451. The promoter at the left end and sequences required for polyadenylation are retained in En-I102. It is transcribed to yield predominantly a 1.8 kb poly(A) RNA. cDNA analysis of this transcript indicated that it contains the coding capacity for a 386 amino acid polypeptide. This polypeptide shares homology with En/Spm encoded functions and we suggest that it interferes with transposition at the protein level.
遗传和分子分析揭示了一种缺失衍生物（En-I102）的特定En元件，它可降低En/Spm诱导的突变率。在En-I102存在的情况下，自主En-1元件和抑制元件Spm-I5719A的切除频率均降低，且切除发生在发育后期。3697 bp长的En-I102元件由En-1通过内部缺失4590 bp（去除核苷酸1862 - 6451）衍生而来。En-I102保留了左端的启动子和多聚腺苷酸化所需的序列。它转录产生主要为1.8 kb的多聚腺苷酸化RNA。对该转录本的cDNA分析表明，它包含编码386个氨基酸多肽的能力。该多肽与En/Spm编码的功能具有同源性，我们认为它在蛋白质水平上干扰转座。
