Design and applications of single and double stranded DNA analogs.

Chemical ligation method for assembling modified DNA-duplexes as well as solid support method and postsynthetic modification in aqueous media was used to synthesize a variety of single and double stranded oligonucleotide derivatives. Novel oligodeoxynucleotides with cluster or alternating sugar modifications and probes resistant to cell nuclease degradation were obtained. The use of such probes for effective and regiospecific hybridase RNA-hydrolysis is provided. Effective synthesis of new DNA duplexes containing modified and chemical cleavable internucleotide bonds was developed. A novel type of affinity reagent was suggested for covalent attachment of substrate to active site nucleophiles in the restriction/modification systems.