Nazarali A J, Gutkind J S, Saavedra J M
Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
J Neurosci Methods. 1989 Dec;30(3):247-53. doi: 10.1016/0165-0270(89)90135-0.
125I-Polymer standards were calibrated by interpolation of their optical densities in [125I]-brain paste standard curves to obtain dpm/mg protein. There was a linear relationship between the calibrated polymer standards and the dpm/mg polymer, as provided by the manufacturer. One dpm/mg polymer was equivalent to 7.34 +/- 0.22 dpm/mg protein. Receptor quantification in selected rat brain areas with comparison to either brain paste or calibrated polymer standards yielded similar results.
通过在[125I] - 脑糊标准曲线中对125I - 聚合物标准品的光密度进行插值来校准，以获得每毫克蛋白质的每分钟衰变数（dpm）。如制造商所提供的，校准后的聚合物标准品与每毫克聚合物的dpm之间存在线性关系。每毫克聚合物的1个dpm相当于每毫克蛋白质7.34±0.22 dpm。与脑糊或校准后的聚合物标准品相比，对选定大鼠脑区的受体定量得出了相似的结果。
