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克隆形成试验与早熟染色体凝集试验在预测人类细胞放射敏感性方面的比较。

Comparison of clonogenic assay with premature chromosome condensation assay in prediction of human cell radiosensitivity.

作者信息

Wang Zhuan-Zi, Li Wen-Jian, Zhang Hong, Yang Jian-She, Qiu Rong, Wang Xiao

机构信息

Graduate School of Chinese Academy of Sciences, Beijing, China.

出版信息

World J Gastroenterol. 2006 Apr 28;12(16):2601-5. doi: 10.3748/wjg.v12.i16.2601.

DOI:10.3748/wjg.v12.i16.2601
PMID:16688809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4087996/
Abstract

AIM

To determine whether the number of non-rejoining G2-chromatid breaks can predict the radiosensitivity of human cell lines.

METHODS

Cell lines of human ovary carcinoma cells (HO8910), human hepatoma cells (HepG2) and liver cells (L02) were irradiated with a range of doses and assessed both of cell survival and non-rejoining G2-chromatid breaks at 24 h after irradiation. Cell survival was documented by a colony assay. Non-rejoining G2-chromatid breaks were measured by counting the number of non-rejoining G2 chromatid breaks at 24 h after irradiation, detected by the prematurely chromosome condensed (PCC) technique.

RESULTS

A linear-quadratic survival curve was observed in three cell lines, and HepG2 was the most sensitive to gamma-radiation. A dose-dependent linear increase was observed in radiation-induced non-rejoining G2-PCC breaks measured at 24 h after irradiation in all cell lines, and HepG2 was the most susceptible to induction of non-rejoining G2-PCC breaks. A close correlation was found between the clonogenic radiosensitivity and the radiation-induced non-rejoining G2-PCC breaks (r = 0.923). Furthermore, survival-aberration correlations for two or more than two doses lever were also significant.

CONCLUSION

The number of non-rejoining G2 PCC breaks holds considerable promise for predicting the radiosensitivity of normal and tumor cells when two or more than two doses lever is tested.

摘要

目的

确定未重新连接的G2期染色单体断裂数量是否可预测人类细胞系的放射敏感性。

方法

用一系列剂量照射人卵巢癌细胞系（HO8910）、人肝癌细胞系（HepG2）和肝细胞系（L02），并在照射后24小时评估细胞存活情况和未重新连接的G2期染色单体断裂情况。通过集落试验记录细胞存活情况。采用早熟染色体凝聚（PCC）技术检测照射后24小时未重新连接的G2期染色单体断裂数量，以此测量未重新连接的G2期染色单体断裂情况。

结果

在三种细胞系中观察到线性二次存活曲线，且HepG2对γ射线最敏感。在所有细胞系中，照射后24小时测量的辐射诱导未重新连接的G2期PCC断裂呈剂量依赖性线性增加，且HepG2最易被诱导产生未重新连接的G2期PCC断裂。克隆形成放射敏感性与辐射诱导未重新连接的G2期PCC断裂之间存在密切相关性（r = 0.923）。此外，两个或两个以上剂量水平的存活-畸变相关性也很显著。

结论

当测试两个或两个以上剂量水平时，未重新连接的G2期PCC断裂数量在预测正常细胞和肿瘤细胞的放射敏感性方面具有很大潜力。

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World J Gastroenterol. 2005 Jul 14;11(26):4098-101. doi: 10.3748/wjg.v11.i26.4098.
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Radioprotective effect of amifostine on cells from cancer prone patients and healthy individuals studied by the G2 and PCC assays.通过G2和早熟染色体凝集试验研究氨磷汀对癌症易感患者和健康个体细胞的辐射防护作用。
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