弓形虫表面抗原在疫苗研发中的生物学作用。
Biological role of surface Toxoplasma gondii antigen in development of vaccine.
作者信息
Liu Ke-Yi, Zhang Dian-Bo, Wei Qing-Kuan, Li Jin, Li Gui-Ping, Yu Jin-Zhi
机构信息
Shandong Institute of Parasitic Diseases, Jining Taibai Zhong Road #11, Jining 272033, Shandong Province, China.
出版信息
World J Gastroenterol. 2006 Apr 21;12(15):2363-8. doi: 10.3748/wjg.v12.i15.2363.
AIM
To analyze the biological role of the surface antigen of Toxoplasma gondii (T gondii) in development of vaccine.
METHODS
The surface antigen of T gondii (SAG1) was expressed in vitro. The immune response of the host to the antigen was investigated by detection of specific antibody reaction to SAG1 and production of cytokines. Mice were immunized with recombinant SAG1 and challenged with lethal strain of T gondii RH. The monoclonal antibody to r-SAG1 was prepared and used to study the effects of SAG1 on T gondii tachyzoites under electromicroscope.
RESULTS
The mice immunized with recombinant SAG1 delayed death for 60 h compared to the control group. The recombinant SAG1 induced specific high titer of IgG and IgM antibodies as well as IFN-gamma, IL-2 and IL-4 cytokines in mice. In contrast, IL-12, IL-6 and TNF-alpha were undetectable. When T gondii tachyzoites were treated with the monoclonal antibody to r-SAG1, the parasites were gathered together, destroyed, deformed, swollen, and holes and gaps formed on the surface.
CONCLUSION
SAG1 may be an excellent vaccine candidate against T gondii. The immune protection induced by SAG1 against T gondii may be regulated by both hormone- and cell-mediated immune response.
目的
分析弓形虫表面抗原在疫苗研发中的生物学作用。
方法
体外表达弓形虫表面抗原（SAG1）。通过检测对SAG1的特异性抗体反应和细胞因子的产生来研究宿主对抗原的免疫反应。用重组SAG1免疫小鼠，并用弓形虫RH致死株进行攻击。制备抗r - SAG1单克隆抗体，并用于在电镜下研究SAG1对弓形虫速殖子的作用。
结果
与对照组相比，用重组SAG1免疫的小鼠死亡延迟60小时。重组SAG1在小鼠中诱导产生特异性高滴度的IgG和IgM抗体以及IFN -γ、IL - 2和IL - 4细胞因子。相反，未检测到IL - 12、IL - 6和TNF -α。当用抗r - SAG1单克隆抗体处理弓形虫速殖子时，寄生虫聚集在一起、被破坏、变形、肿胀，表面形成孔洞和缝隙。
结论
SAG1可能是一种优秀的抗弓形虫疫苗候选物。SAG1诱导的针对弓形虫的免疫保护可能受激素介导和细胞介导的免疫反应调节。
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