Department of Microbiology and Immunology, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China.
Parasitol Res. 2011 Sep;109(3):637-44. doi: 10.1007/s00436-011-2296-5. Epub 2011 Mar 15.
The aim of this work was to evaluate immune responses in BALB/c mice vaccinated subcutaneously by recombinant protein, or intramuscularly by plasmid DNA with fusion antigen of rhoptry protein 2 (ROP2) and major surface protein 1 (SAG1) from Toxoplasma gondii (T. gondii). BALB/c mice were immunized with one of three different antigen formulations respectively, which were rROP2-SAG1, pcROP2-SAG1, and pcROP2-SAG1 boosted with rROP2-SAG1. The production of IgG, IgG subclasses, lymphoproliferation, and level of gamma interferon (IFN-γ) were detected after vaccination. The animals vaccinated with rROP2-SAG1 quickly developed specific anti-TLA (T. gondii lysate antigen) antibodies, which continued to rise after immunization. However, production of IgG against TLA in mice vaccinated with pcROP2-SAG1 was relatively slow and maintained a high level after reaching plateau. There are more vigorous specific lymphoproliferative responses observed in mice of group rROP2-SAG1 than in pcROP2-SAG1. Immune responses in mice of group pcROP2-SAG1 boosted with rROP2-SAG1 were similar to the protein immunization group. Three immunization procedures resulted in a similar level of IFN-γ production. Our results indicate that BALB/c mice vaccinated by three immunization procedures induce similar humoral and cellular immunity against infection of T. gondii. Mice immunized with recombinant protein rROP2-SAG1 produce more humoral immune responses than mice immunized with other antigen formulations.
本研究旨在评估皮下注射重组蛋白或肌肉内注射融合抗原 rhoptry 蛋白 2（ROP2）和主要表面蛋白 1（SAG1）的 BALB/c 小鼠的免疫反应。BALB/c 小鼠分别用三种不同的抗原制剂免疫，分别为 rROP2-SAG1、pcROP2-SAG1 和 pcROP2-SAG1 加强 rROP2-SAG1。免疫后检测 IgG、IgG 亚类、淋巴细胞增殖和γ干扰素（IFN-γ）水平。用 rROP2-SAG1 免疫的动物迅速产生针对 TLA（弓形虫裂解抗原）的特异性抗体，免疫后持续升高。然而，用 pcROP2-SAG1 免疫的小鼠产生针对 TLA 的 IgG 相对较慢，达到平台期后仍保持高水平。rROP2-SAG1 组小鼠的特异性淋巴增殖反应比 pcROP2-SAG1 组更强烈。用 rROP2-SAG1 加强 pcROP2-SAG1 的免疫小鼠的免疫反应与蛋白免疫组相似。三种免疫程序导致 IFN-γ产生水平相似。我们的结果表明，三种免疫程序接种的 BALB/c 小鼠诱导了针对弓形虫感染的相似的体液和细胞免疫。用重组蛋白 rROP2-SAG1 免疫的小鼠比用其他抗原制剂免疫的小鼠产生更多的体液免疫反应。
