Vidal E, Márquez M, Costa C, Tortosa R, Domènech A, Serafín A, Pumarola M
Laboratori PRIOCAT, Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
Vet J. 2007 Jul;174(1):196-9. doi: 10.1016/j.tvjl.2006.03.007. Epub 2006 May 11.
Molecular profiling of the proteinase K resistant prion protein (PrP(res)) is a technique that has been applied to the characterisation of transmissible spongiform encephalopathy (TSE) strains. An interesting example of the application of this technique is the ability to differentiate, at the experimental level, between bovine spongiform encephalopathy (BSE) and scrapie infection in sheep, and to distinguish between classical and atypical BSE and scrapie cases. Twenty-six BSE cases and two scrapie cases from an active TSE surveillance program and diagnosed at the PRIOCAT, TSE Reference Laboratory (Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona, Catalunya, Spain) were examined by Western blotting. Molecular profiling was achieved by comparing the glycosylation profile, deglycosylated PrP molecular weight and 6H4/P4 monoclonal antibody binding ratio. The results obtained during the characterisation of these field cases indicated an absence of atypical BSE cases in Catalunya.
蛋白酶K抗性朊病毒蛋白（PrP(res)）的分子谱分析是一种已应用于传染性海绵状脑病（TSE）毒株特征鉴定的技术。该技术应用的一个有趣例子是，在实验水平上能够区分牛海绵状脑病（BSE）和绵羊痒病感染，并区分经典型和非典型BSE以及痒病病例。对来自一个活跃的TSE监测项目、在PRIOCAT的TSE参考实验室（西班牙加泰罗尼亚巴塞罗那自治大学动物卫生研究中心）诊断的26例BSE病例和2例痒病病例进行了蛋白质印迹检测。通过比较糖基化谱、去糖基化PrP分子量和6H4/P4单克隆抗体结合率来实现分子谱分析。在这些现场病例的特征鉴定过程中获得的结果表明，加泰罗尼亚不存在非典型BSE病例。
