Deeg Cornelia A, Pompetzki Dirk, Raith Albert J, Hauck Stefanie M, Amann Barbara, Suppmann Sabine, Goebel Thomas W F, Olazabal Ursula, Gerhards Hartmut, Reese Sven, Stangassinger Manfred, Kaspers Bernd, Ueffing Marius
Institute of Animal Physiology, Ludwig Maximilians University (LMU) Munich, Veterinärstr. 13, D-80539 Munich, Germany.
Mol Cell Proteomics. 2006 Aug;5(8):1462-70. doi: 10.1074/mcp.M500352-MCP200. Epub 2006 May 11.
The development, progression, and recurrence of autoimmune diseases are frequently driven by a group of participatory autoantigens. We identified and characterized novel autoantigens by analyzing the autoantibody binding pattern from horses affected by spontaneous equine recurrent uveitis to the retinal proteome. Cellular retinaldehyde-binding protein (cRALBP) had not been described previously as autoantigen, but subsequent characterization in equine recurrent uveitis horses revealed B and T cell autoreactivity to this protein and established a link to epitope spreading. We further immunized healthy rats and horses with cRALBP and observed uveitis in both species with typical tissue lesions at cRALBP expression sites. The autoantibody profiling outlined here could be used in various autoimmune diseases to detect autoantigens involved in the dynamic spreading cascade or serve as predictive markers.
自身免疫性疾病的发生、发展和复发通常由一组参与的自身抗原驱动。我们通过分析自发性马复发性葡萄膜炎患马的自身抗体与视网膜蛋白质组的结合模式，鉴定并表征了新型自身抗原。细胞视黄醛结合蛋白（cRALBP）此前未被描述为自身抗原，但随后在马复发性葡萄膜炎患马中的表征揭示了针对该蛋白的B细胞和T细胞自身反应性，并建立了与表位扩展的联系。我们进一步用cRALBP免疫健康大鼠和马，在两个物种中均观察到葡萄膜炎，且在cRALBP表达位点出现典型的组织病变。本文概述的自身抗体分析可用于各种自身免疫性疾病，以检测参与动态扩展级联反应的自身抗原或作为预测标志物。
