Simeonova Galina P, Krastev Svetozar Z, Simeonov Radostin S
Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.
Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.
Vet Res Commun. 2016 Dec;40(3-4):107-115. doi: 10.1007/s11259-016-9659-4. Epub 2016 Jun 25.
The pathogenic mechanism of equine recurrent uveitis (ERU) is still poorly defined and many variations between experimental animal models and spontaneous disease exist.
The aim of our study was to investigate if Th17 cell-mediated response plays role in the pathogenesis of the used experimental model in horses and to reveal its pathological findings.
Experimental uveitis was induced in 6 healthy horses. The concentrations of retinal autoantigen CRALBP and IL-17 were measured using ELISA in aqueous humor and vitreous body of the 12 inflamed eyes as well as in 12 control non-inflamed eyes taken from 6 horses in slaughter house. After centrifugation of the two eye media, smears were prepared and cytological investigation was performed. Tissue specimens were taken from all eye globes and were submitted to histopathological investigation.
CRALBP and IL-17 concentrations were significantly elevated in eye media of horses with experimental uveitis in comparison with controls. Cytological and histopathological findings corresponded to the changes characteristic of chronic immune-mediated inflammation with mononuclear cell infiltration of uvea, choroid, retina, and eye media as well as severe retinal destruction.
Our study demonstrated the involvement of the retinal autoantigen CRALBP as well as IL-17 in the pathogenesis of experimental uveitis in horses. These findings suggests that this experimental uveitis in horses may serve as a suitable animal model for investigation of IL-17- mediated immune response during spontaneous autoimmune uveitis in horses as well as in humans.
马复发性葡萄膜炎（ERU）的致病机制仍未完全明确，实验动物模型与自然发病之间存在许多差异。
本研究旨在探讨Th17细胞介导的反应是否在所用马实验模型的发病机制中起作用，并揭示其病理表现。
对6匹健康马诱发实验性葡萄膜炎。采用酶联免疫吸附测定法（ELISA）检测12只发炎眼睛的房水和玻璃体以及从屠宰场的6匹马获取的12只对照未发炎眼睛中视网膜自身抗原CRALBP和白细胞介素-17（IL-17）的浓度。对两种眼内介质进行离心后，制备涂片并进行细胞学检查。从所有眼球获取组织标本并进行组织病理学检查。
与对照组相比，实验性葡萄膜炎马的眼内介质中CRALBP和IL-17浓度显著升高。细胞学和组织病理学结果与慢性免疫介导炎症的特征性变化相符，包括葡萄膜、脉络膜、视网膜和眼内介质的单核细胞浸润以及严重的视网膜破坏。
我们的研究证明视网膜自身抗原CRALBP以及IL-17参与了马实验性葡萄膜炎的发病机制。这些发现表明，这种马实验性葡萄膜炎可能作为一种合适的动物模型，用于研究马以及人类自发性自身免疫性葡萄膜炎期间IL-17介导的免疫反应。
