Steven F S, Zoeller J, Maier H, Born I A
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
J Enzyme Inhib. 1991;5(2):151-64. doi: 10.3109/14756369109069067.
The epithelial cells in squamous carcinoma and leukoplakia of the oral cavity possess the cell surface protease, guanidinobenzoatase (GB), in an active form. GB is closely similar to plasminogen activator, a protease associated with both transformed cells and tumour cells. The active centre of GB binds the fluorescent probe 9-aminoacridine (9-AA) enabling cells containing active GB to be visualised by fluorescent microscopy. It was observed that chemotherapy with cisplatin resulted in a marked decrease in cell surface GB activity and this decrease was due to the formation of an enzyme-inhibitor complex. One of the results of chemotherapy was shown to be the suppression of a cell surface protease which is known to be associated with migration and malignancy of cells in vivo.
口腔鳞状细胞癌和白斑中的上皮细胞具有活性形式的细胞表面蛋白酶——胍基苯甲酰酶（GB）。GB与纤溶酶原激活剂极为相似，纤溶酶原激活剂是一种与转化细胞和肿瘤细胞都相关的蛋白酶。GB的活性中心能结合荧光探针9-氨基吖啶（9-AA），使得含有活性GB的细胞能够通过荧光显微镜观察到。据观察，顺铂化疗导致细胞表面GB活性显著降低，这种降低是由于形成了一种酶-抑制剂复合物。化疗的结果之一是抑制了一种细胞表面蛋白酶，已知该蛋白酶与体内细胞的迁移和恶性肿瘤有关。
