Steven F S, Griffin M M, Zoeller J, Maier H
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
Anticancer Res. 1990 Jul-Aug;10(4):995-9.
Squamous cell carcinoma cells possess a cell surface protease referred to as guanidinobenzoatase (GB) which is very similar to the single chain form of tissue type plasminogen activator. This enzyme binds fluorescent probes at its active center and cells possessing GB can be distinguished from those that lack this enzyme by fluorescent microscopic techniques. Normal squamous epithelial cells shed from the surface of the oral cavity lack GB and do not exhibit cell surface fluorescence when pretreated with such fluorescent probes. We have used this knowledge to design a simple technique for the rapid location of squamous cell carcinoma cells in oral smears; our results are presented in the form of colour prints in which the tumour cells can be easily distinguished from other cells by their fluorescence.
鳞状细胞癌细胞具有一种细胞表面蛋白酶，称为胍基苯甲酸酶（GB），它与组织型纤溶酶原激活剂的单链形式非常相似。这种酶在其活性中心结合荧光探针，拥有GB的细胞可以通过荧光显微镜技术与缺乏这种酶的细胞区分开来。从口腔表面脱落的正常鳞状上皮细胞缺乏GB，在用这种荧光探针预处理时不会表现出细胞表面荧光。我们利用这一知识设计了一种简单的技术，用于在口腔涂片快速定位鳞状细胞癌细胞；我们的结果以彩色印刷品的形式呈现，其中肿瘤细胞因其荧光可轻易与其他细胞区分开来。
