小鼠肝细胞核中NAD-焦磷酸化酶活性的超微结构证明
Ultrastructural demonstration of NAD-pyrophosphorylase activity in mouse liver nuclei.
作者信息
Unger E, Buchwalow I B, Hartmann M, Kleschtschinow W M, Raikhlin N T
出版信息
Histochemistry. 1975;43(1):73-9. doi: 10.1007/BF00490156.
Ultrastructural demonstration of NAD-pyrophosphorylase activity (E.C.2.7.7.1) in isolated mouse liver nuclei was investigated with the use of an electronhistochemical procedure based on the precipitation of pyrophosphate ions with lead ions under conditions permitting simultaneous ATPase inhibition by formaldehyde/ethanol prefixation. In isolated mouse liver nuclei activity of NAD-pyrophosphorylase was found in nucleoli, in interchromatin granules, coiled bodies and strand-like structures in nucleoplasm.
利用一种基于在甲醛/乙醇预固定可同时抑制ATP酶的条件下，焦磷酸离子与铅离子沉淀的电子组织化学方法，对分离的小鼠肝细胞核中NAD-焦磷酸化酶活性（E.C.2.7.7.1）进行了超微结构显示研究。在分离的小鼠肝细胞核中，发现NAD-焦磷酸化酶活性存在于核仁、染色质间颗粒、卷曲小体和核质中的丝状结构中。
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