Yu Zhi-Hong, Wei Pin-Kang, Xu Ling, Qin Zhi-Feng, Shi Jun, Xiao Yan, Lin Hui-Ming
Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Zhong Xi Yi Jie He Xue Bao. 2006 May;4(3):275-80. doi: 10.3736/jcim20060311.
To assess the effects of Jinlongshe Granules (JLSG) on tumor growth of gastric carcinoma.
Fifty nude mice orthotopically transplanted with MKN-45 human gastric cancer cells were divided into five groups: untreated group, 5-fluorouracil (5-FU)-treated group and high-, medium-, and low-dose JLSG-treated groups. Corresponding antitumor drugs were administered in each group except the untreated group. The antitumor effects in vivo were evaluated. Cell cycle distribution and apoptosis of MKN-45 human gastric cancer cells were determined by using flow cytometry (FCM) and Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) staining assay. The ultrastructure of MKN-45 gastric cancer cells was observed by transmission electron microscope.
In the mice treated with high-, medium-, and low-dose JLSG, the growth inhibition rates of gastric cancer were 68.13%, 55.94% and 50.31% respectively, and this antitumor effect was dose-dependent. In the mice treated with intraperitoneal injection of 5-FU, the growth inhibition rate of gastric cancer was 53.43% and not much different from those treated with JLSG. The apoptotic rates in the high-, medium-, and low-dose JLSG-treated groups were 22.81%, 28.27% and 38.54% respectively, in a dose-dependent manner, with the cell cycle arrested at G(0)/G(1) phase. An Annexin V-FITC/PI staining assay revealed that the percentages of early apoptotic cells in the three dose JLSG-treated groups were all significantly higher than that in the 5-FU-treated group, whereas the late apoptotic and necrotic cells were much more in the 5-FU-treated group than those in the three dose JLSG-treated groups.
Jinlongshe Granules exert an inhibiting effect on MKN-45 human gastric cancer cell.
评估金龙蛇颗粒（JLSG）对胃癌肿瘤生长的影响。
将50只原位移植人MKN - 45胃癌细胞的裸鼠分为五组：未治疗组、5 - 氟尿嘧啶（5 - FU）治疗组以及高、中、低剂量JLSG治疗组。除未治疗组外，每组给予相应的抗肿瘤药物。评估体内抗肿瘤效果。采用流式细胞术（FCM）和膜联蛋白V - 异硫氰酸荧光素/碘化丙啶（Annexin V - FITC/PI）染色法检测MKN - 45人胃癌细胞的细胞周期分布和凋亡情况。通过透射电子显微镜观察MKN - 45胃癌细胞的超微结构。
高、中、低剂量JLSG治疗的小鼠中，胃癌生长抑制率分别为68.13%、55.94%和50.31%，且这种抗肿瘤作用呈剂量依赖性。腹腔注射5 - FU治疗的小鼠中，胃癌生长抑制率为53.43%，与JLSG治疗组差异不大。高、中、低剂量JLSG治疗组的凋亡率分别为22.81%、28.27%和38.54%，呈剂量依赖性，细胞周期阻滞于G(0)/G(1)期。Annexin V - FITC/PI染色分析显示，三个剂量JLSG治疗组的早期凋亡细胞百分比均显著高于5 - FU治疗组，而5 - FU治疗组的晚期凋亡和坏死细胞比三个剂量JLSG治疗组更多。
金龙蛇颗粒对MKN - 45人胃癌细胞具有抑制作用。
