Guo Xiao-dong, Wei Pin-kang
Department of Oncology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Zhong Xi Yi Jie He Xue Bao. 2007 Jul;5(4):432-6. doi: 10.3736/jcim20070414.
To observe the influence of Xiaotan Sanjie Recipe on growth of the transplanted tumor and expressions of proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGFR) in tissue of the gastric carcinoma of nude mice, and to explore the mechanism of Xiaotan Sanjie Recipe in restraining the multiplication of the stomach cancer.
MKN-45 gastric carcinoma model in nude mice was established. Forty-five nude mice were randomly divided into control group, Xiaotan Sanjie group and 5-fluorouracil (5-FU) group. Drugs were given on the next day of inoculation. Mice in the control group were given normal saline, and mice in the Xiaotan Sanjie group were given Xiaotan Sanjie Recipe. Intraperitoneal injection of 5-FU was administered in the 5-FU group. The expressions of PCNA and EGFR were examined by using streptavidin peroxidase (SP) conjugate technique, and the tumor weight and pathological characteristics were also observed.
Compared with the control group, Xiaotan Sanjie Recipe significantly restrained the growth of the transplanted tumor, and reduced the expressions of PCNA and EGFR in tissue of the gastric carcinoma of nude mice (P<0.05).
Xiaotan Sanjie Recipe can disturb the synthesis of DNA and reduce the proliferous activity of cancer cells by decreasing the expressions of PCNA and EGFR.
观察消痰散结方对裸鼠胃癌移植瘤生长及癌组织中增殖细胞核抗原（PCNA）和表皮生长因子受体（EGFR）表达的影响，探讨消痰散结方抑制胃癌增殖的作用机制。
建立裸鼠MKN-45胃癌模型。45只裸鼠随机分为对照组、消痰散结组和5-氟尿嘧啶（5-FU）组。接种次日开始给药，对照组给予生理盐水，消痰散结组给予消痰散结方，5-FU组腹腔注射5-FU。采用链霉亲和素-过氧化物酶（SP）免疫组化法检测PCNA和EGFR的表达，并观察肿瘤重量及病理特征。
与对照组比较，消痰散结方可显著抑制裸鼠胃癌移植瘤生长，降低癌组织中PCNA和EGFR的表达（P<0.05）。
消痰散结方可能通过降低PCNA和EGFR的表达来干扰DNA合成，降低癌细胞的增殖活性。
