Wilhelm Vivian, Miquel Alvaro, Burzio Luis O, Rosemblatt Mario, Engel Esteban, Valenzuela Sebastián, Parada Gustavo, Valenzuela Pablo D T
Fundación Ciencia para la Vida and Instituto Milenio de Biología Fundamental y Aplicada, Zañartu 1482, Santiago, Chile.
Vaccine. 2006 Jun 5;24(23):5083-91. doi: 10.1016/j.vaccine.2006.03.027. Epub 2006 Mar 29.
We report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to select optimal candidates for a recombinant subunit vaccine to protect fish from rickettsial septicaemia (SRS). Based on this information, 15 P. salmonis ORFs encoding heat shock proteins, virulence factors, membrane bound and other surface exposed antigens, were isolated and expressed. Seven of the most promising antigens were formulated in three mixtures (V1-V3) containing two or three recombinant proteins each and injected into salmon to test their protective efficacy. Two of the three formulations (V1, V2) elicited a strong protective response in a challenge against the pathogen, which was coincident with the humoral response against the corresponding recombinant proteins present in each formulation. V1, formulated with recombinant chaperonines Hsp60, Hsp70 and flagellar protein FlgG of P. salmonis achieved the highest level of protection with a relative percent survival (RPS) of 95%.
我们在此报告重组蛋白混合物对鱼类里氏杆菌病的保护作用。对鲑居尾孢菌基因组文库采用比较基因组学策略，以选择用于重组亚单位疫苗的最佳候选物，保护鱼类免受立克次氏体败血症（SRS）。基于此信息，分离并表达了15个编码热休克蛋白、毒力因子、膜结合和其他表面暴露抗原的鲑居尾孢菌开放阅读框。将七个最有前景的抗原配制成三种混合物（V1 - V3），每种混合物包含两种或三种重组蛋白，并注射到鲑鱼中以测试其保护效果。三种制剂中的两种（V1、V2）在针对病原体的攻毒试验中引发了强烈的保护反应，这与针对每种制剂中相应重组蛋白的体液反应一致。由鲑居尾孢菌的重组伴侣蛋白Hsp60、Hsp70和鞭毛蛋白FlgG配制的V1实现了最高水平的保护，相对存活率（RPS）为95%。
