Ramachandran Ramesh, Ocón-Grove Olga M, Metzger Shana L
Department of Poultry Science, The Pennsylvania State University, University Park, PA 16802, USA.
Domest Anim Endocrinol. 2007 Jul;33(1):19-31. doi: 10.1016/j.domaniend.2006.04.004. Epub 2006 May 2.
AdipoR1 and AdipoR2 belong to a novel class of transmembrane receptors that mediate the effects of adiponectin. We have cloned the chicken AdipoR1 and AdipoR2 complementary deoxyribonucleic acids (cDNA) and determined their expression in various tissues. We also investigated the effect of feed deprivation on the expression of AdipoR1 or AdipoR2 mRNA in the chicken diencephalon, liver, anterior pituitary gland, and adipose tissue. The chicken AdipoR1 and AdipoR2 cDNA sequences were 76-83% identical to the respective mammalian sequences. A hydrophobicity analysis of the deduced amino acid sequences of chicken AdipoR1/AdipoR2 revealed seven distinct hydrophobic regions representing seven transmembrane domains. By RT-PCR, we detected AdipoR1 and AdipoR2 mRNA in adipose tissue, liver, anterior pituitary gland, diencephalon, skeletal muscle, kidney, spleen, ovary, and blood. AdipoR1 or AdipoR2 mRNA expression in various tissues was quantified by real-time quantitative PCR, and AdipoR1 mRNA expression was the highest in skeletal muscle, adipose tissue and diencephalon, followed by kidney, ovary, liver, anterior pituitary gland, and spleen. AdipoR2 mRNA expression was the highest in adipose tissue followed by skeletal muscle, liver, ovary, diencephalon, anterior pituitary gland, kidney, and spleen. We also found that a 48 h feed deprivation significantly decreased AdipoR1 mRNA quantity in the chicken pituitary gland, while AdipoR2 mRNA quantity was significantly increased in adipose tissue (P<0.05). We conclude that the AdipoR1 and AdipoR2 genes are ubiquitously expressed in chicken tissues and that their expression is altered by feed deprivation in the anterior pituitary gland and adipose tissue.
脂联素受体1（AdipoR1）和脂联素受体2（AdipoR2）属于一类新型跨膜受体，介导脂联素的作用。我们克隆了鸡的AdipoR1和AdipoR2互补脱氧核糖核酸（cDNA），并确定了它们在各种组织中的表达情况。我们还研究了禁食对鸡间脑、肝脏、垂体前叶和脂肪组织中AdipoR1或AdipoR2 mRNA表达的影响。鸡的AdipoR1和AdipoR2 cDNA序列与各自的哺乳动物序列有76 - 83%的同源性。对鸡AdipoR1/AdipoR2推导的氨基酸序列进行的疏水性分析显示有七个不同的疏水区，代表七个跨膜结构域。通过逆转录聚合酶链反应（RT-PCR），我们在脂肪组织、肝脏、垂体前叶、间脑、骨骼肌、肾脏、脾脏、卵巢和血液中检测到了AdipoR1和AdipoR2 mRNA。通过实时定量聚合酶链反应对各种组织中AdipoR1或AdipoR2 mRNA的表达进行定量，AdipoR1 mRNA在骨骼肌、脂肪组织和间脑中表达最高，其次是肾脏、卵巢、肝脏、垂体前叶和脾脏。AdipoR2 mRNA在脂肪组织中表达最高，其次是骨骼肌、肝脏、卵巢、间脑、垂体前叶、肾脏和脾脏。我们还发现，禁食48小时显著降低了鸡垂体中AdipoR1 mRNA的量，而脂肪组织中AdipoR2 mRNA的量显著增加（P<0.05）。我们得出结论，AdipoR1和AdipoR2基因在鸡组织中广泛表达，并且它们的表达在前叶垂体和脂肪组织中会因禁食而改变。
