Benoit Isabelle, Navarro David, Marnet Nathalie, Rakotomanomana Nnjara, Lesage-Meessen Laurence, Sigoillot Jean-Claude, Asther Marcel, Asther Michèle
UMR-1163 INRA de Biotechnologie des Champignons Filamenteux, IFR86-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
Carbohydr Res. 2006 Aug 14;341(11):1820-7. doi: 10.1016/j.carres.2006.04.020. Epub 2006 May 15.
Agro-industrial by-products are a potential source of added-value phenolic acids with promising applications in the food and pharmaceutical industries. Here two purified feruloyl esterases from Aspergillus niger, FAEA and FAEB were tested for their ability to release phenolic acids such as caffeic acid, p-coumaric acid and ferulic acid from coffee pulp, apple marc and wheat straw. Their hydrolysis activity was evaluated and compared with their action on maize bran and sugar beet pulp. The specificity of both enzymes against natural and synthetic substrates was evaluated; particular attention was paid to quinic esters and lignin monomers. The efficiency of both enzymes on model substrates was studied. We show the ability of these enzymes to hydrolyze quinic esters and ester linkages between phenolic acids and lignin monomer.
农产品加工副产品是具有附加值的酚酸的潜在来源，在食品和制药行业有着广阔的应用前景。本文对两种来自黑曲霉的纯化阿魏酸酯酶FAEA和FAEB从咖啡渣、苹果渣和麦秸中释放酚酸（如咖啡酸、对香豆酸和阿魏酸）的能力进行了测试。评估了它们的水解活性，并与它们对玉米麸皮和甜菜渣的作用进行了比较。评估了这两种酶对天然和合成底物的特异性；特别关注了奎尼酸酯和木质素单体。研究了这两种酶在模型底物上的效率。我们展示了这些酶水解奎尼酸酯以及酚酸与木质素单体之间酯键的能力。
