Ninfali P, Bacchiocca M, Antonelli A, Biagiotti E, Di Gioacchino A M, Piccoli G, Stocchi V, Brandi G
Istituto di Chimica Biologica G. Fornaini, Università degli Studi Carlo Bo, Urbino (PU), Italy.
Phytomedicine. 2007 Feb;14(2-3):216-21. doi: 10.1016/j.phymed.2006.03.006. Epub 2006 May 15.
The molecular components of a phenolic fraction (P2), obtained from liquid chromatography of a Swiss Chard (Beta vulgaris subsp. cycla) extract, were identified using HPLC-ESI-MS/MS. The primary P2 components were: vitexin-2''O-rhamnoside, its demethylated form 2''-xylosylvitexin, isorhamnetin 3-gentiobioside, and rutin. P2 "in toto" and the single components were characterized for antioxidant capacity, antimitotic activity on MCF-7 human breast cancer cells and for toxicity to human lymphocytes and macrophages. P2 inhibited MCF-7 cell proliferation (IC(50) value = 9 microg/ml) without inducing apoptosis, showed no toxicity to human lymphocytes and slight toxicity to macrophages. Vitexin-2''O-rhamnoside strongly inhibited DNA synthesis in MCF-7 cells, whereas 2''-xylosylvitexin and isorhamnetin 3-gentiobioside were activators; combinations of activators and inhibitors maintained the over-all inhibitory effect.
通过对瑞士甜菜（Beta vulgaris subsp. cycla）提取物进行液相色谱分析得到酚类组分（P2），利用高效液相色谱-电喷雾串联质谱（HPLC-ESI-MS/MS）对其分子成分进行鉴定。P2的主要成分有：牡荆素-2''-O-鼠李糖苷、其去甲基化形式2''-木糖基牡荆素、异鼠李素3-龙胆二糖苷和芦丁。对P2整体及单一成分的抗氧化能力、对MCF-7人乳腺癌细胞的抗有丝分裂活性以及对人淋巴细胞和巨噬细胞的毒性进行了表征。P2抑制MCF-7细胞增殖（半数抑制浓度（IC50）值 = 9微克/毫升）且不诱导细胞凋亡，对人淋巴细胞无毒性，对巨噬细胞有轻微毒性。牡荆素-2''-O-鼠李糖苷强烈抑制MCF-7细胞中的DNA合成，而2''-木糖基牡荆素和异鼠李素3-龙胆二糖苷是激活剂；激活剂和抑制剂的组合保持了总体抑制作用。
