Arabiun Hamidreza, Bordbar Hossein, Dehghani Nazhvani Seifollah, Ebrahimi Reyhaneh, Aliabadi Ehsan, Ghanbari Ilnaz
Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Dept. of Anatomical Sciences, Histomorphometry, Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
J Dent (Shiraz). 2020 Sep;21(3):225-233. doi: 10.30476/DENTJODS.2020.81808.0.
Autogenous bone graft is the gold standard for bone reconstruction. Osteogenic cells must be kept viable in graft for a successful procedure. In extracorporeal preservation of grafts during surgery, three different factors may influence the quality of grafts. These factors include temperature, storage medium, and time interval.
In this study, we evaluated the effects of different storage media, temperatures, and times on osteoblast count in autogenous bone grafts, preserved extracorporeally.
Samples were obtained from iliac crest region in a goat. The grafts were preserved in 36 groups of different storage time, temperature, and medium. Samples were histomorphometrically analyzed to determine osteoblast count as the criteria of graft quality.
In almost all samples, room temperature was the most and incubator was the least favorable storage temperatures. In grafts preserved in room temperature, no difference was noted between normal saline and Ringer's lactate solution and in almost all of the samples autologous blood and dry environment were more favorable media than Ringer's lactate solution. The effect of storage time was highly depended on the combination of temperature and solution.
The results demonstrated that for preserving as many osteoblasts as possible in bone grafts, the best temperature was room temperature and the least favorable temperature was incubator. In addition, when bone fragments were preserved in room temperature, the best medium for graft storage was blood, which showed better results than normal saline and Ringer's lactate solution.
自体骨移植是骨重建的金标准。成骨细胞必须在移植物中保持存活才能使手术成功。在手术过程中对移植物进行体外保存时，有三个不同因素可能会影响移植物的质量。这些因素包括温度、储存介质和时间间隔。
在本研究中，我们评估了不同储存介质、温度和时间对体外保存的自体骨移植物中成骨细胞数量的影响。
从山羊的髂嵴区域获取样本。移植物保存在36组不同的储存时间、温度和介质中。对样本进行组织形态计量学分析，以确定成骨细胞数量作为移植物质量的标准。
在几乎所有样本中，室温是最适宜的储存温度，培养箱是最不适宜的储存温度。在室温保存的移植物中，生理盐水和乳酸林格液之间没有差异，并且在几乎所有样本中，自体血和干燥环境比乳酸林格液更适宜作为储存介质。储存时间的影响高度依赖于温度和溶液的组合。
结果表明，为了在骨移植物中尽可能多地保存成骨细胞，最佳温度是室温，最不适宜的温度是培养箱温度。此外，当骨碎片在室温下保存时，移植物储存的最佳介质是血液，其效果优于生理盐水和乳酸林格液。
